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PMID: 3478552 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato.

Molecular & general genetics : MGG ·Vol. 209 ·No. 2 ·1987-09-00 ·Pages 247-56

Sugita M, Manzara T, Pichersky E, Cashmore A, Gruissem W

Abstract

We have cloned and sequenced all five members of the gene family for the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato, Lycopersicon esculentum cv. VFNT LA 1221 cherry line. Two of the five genes, designated Rbcs-1 and Rbcs-2, are present as single genes at individual loci. Three genes, designated Rbcs-3A, Rbcs-3B and Rbcs-3C, are organized in a tandem array within 10 kb at a third independent locus. The Rbcs-2 gene contains three introns; all the other members of the tomato gene family contain two introns. The coding sequence of Rbcs-1 differs by 14.0% from that of Rbcs-2 and by 13.3% from that of Rbcs-3 genes. Rbcs-2 shows 10.4% divergence from Rbcs-3. The exon and intron sequences of Rbcs-3A are identical to those of Rbcs-3C, and differ by 1.9% from those of Rbcs-3B. Nucleotide sequence analysis suggests that the five rbcS genes encode four different precursors, and three different mature polypeptides. S1 nuclease mapping of the 5' end of rbcS mRNAs revealed that the mRNA leader sequences vary in length from 8 to 75 nucleotides. Northern analysis using gene-specific oligonucleotide probes from the 3' non-coding region of each gene reveals a four to five-fold difference among the five genes in maximal steady-state mRNA levels in leaves.

MeSH Terms
Amino Acid Sequence Base Sequence Genes Macromolecular Substances Molecular Sequence Data Nucleic Acid Hybridization Plants/enzymology Ribulose-Bisphosphate Carboxylase/genetics Transcription, Genetic
Chemicals
Macromolecular Substances Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sugita M
Department of Botany, University of California, Berkeley 94720.
Manzara T
Pichersky E
Cashmore A
Gruissem W
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329650
Published
1987-09-00
Pages
247-56
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM33813 · United States
External Links
PubMed source DOI: 10.1007/BF00329650
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