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PMID: 3485286 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete sequence of HLA-B27 cDNA identified through the characterization of structural markers unique to the HLA-A, -B, and -C allelic series.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 5 ·1986-03-00 ·Pages 1428-32

Szöts H, Riethmüller G, Weiss E, Meo T

Abstract

Antigen HLA-B27 is a high-risk genetic factor with respect to a group of rheumatoid disorders, especially ankylosing spondylitis. A cDNA library was constructed from an autozygous B-cell line expressing HLA-B27, HLA-Cw1, and the previously cloned HLA-A2 antigen. Clones detected with an HLA probe were isolated and sorted into homology groups by differential hybridization and restriction maps. Nucleotide sequencing allowed the unambiguous assignment of cDNAs to HLA-A, -B, and -C loci. The HLA-B27 mRNA has the structural features and the codon variability typical of an HLA class I transcript but it specifies two uncommon amino acid replacements: a cysteine in position 67 and a serine in position 131. The latter substitution may have functional consequences, because it occurs in a conserved region and at a position invariably occupied by a species-specific arginine in humans and lysine in mice. The availability of the complete sequence of HLA-B27 and of the partial sequence of HLA-Cw1 allows the recognition of locus-specific sequence markers, particularly, but not exclusively, in the transmembrane and cytoplasmic domains.

MeSH Terms
Base Sequence DNA/genetics HLA Antigens/genetics HLA-B27 Antigen HLA-C Antigens Humans Major Histocompatibility Complex RNA, Messenger/genetics
Chemicals
HLA Antigens HLA-B27 Antigen HLA-C Antigens HLA-Cw1 antigen RNA, Messenger DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Szöts H
Riethmüller G
Weiss E
Meo T
References (33)
33 references, click to expand
  1. Evolutionary significance of the HL-A system.
    Nature. 1972 May 19;237(5351):139-45 passim PMID: 4113158
  2. Structure of crossreactive human histocompatibility antigens HLA-A28 and HLA-A2: possible implications for the generation of HLA polymorphism.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3813-7 PMID: 6179086
  3. Mixed lymphocyte cultures with inbred individuals: an approach to MLC typing.
    Tissue Antigens. 1973;3(4):323-9 PMID: 4272111
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. HLA--D typing with lymphoblastoid cell lines. IV. Allelic relationships.
    Tissue Antigens. 1979 Jan;13(1):35-44 PMID: 84414
  7. Comparison of amino acid sequences of two human histocompatibility antigens, HLA-A2 and HLA-B7: location of putative alloantigenic sites.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4395-9 PMID: 92029
  8. Primary structure of the H-2Db alloantigen. II. Additional amino acid sequence information, localization of a third site of glycosylation and evidence for K and D region specific sequences.
    Immunogenetics. 1982;16(1):11-22 PMID: 7118212
  9. Subtypes of HLA-B27 detected by cytotoxic T lymphocytes and their role in self-recognition.
    Hum Immunol. 1982 Dec;5(4):259-68 PMID: 6186653
  10. Biochemical analysis of variant HLA-B27 antigens.
    Hum Immunol. 1983 Feb;6(2):111-7 PMID: 6601098
  11. Sequences of human repetitive DNA, non-alpha-globin genes, and major histocompatibility locus genes.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1079-86 PMID: 6574860
  12. A directional process of gene conversion is expected to yield dynamic polymorphism associated with stability of alternative alleles in class I histocompatibility antigens gene family.
    Biochimie. 1983 Apr-May;65(4-5):229-37 PMID: 6409157
  13. Primary structure of papain-solubilized human histocompatibility antigen HLA-B40 (-Bw60). An outline of alloantigenic determinants.
    Biochemistry. 1983 Aug 2;22(16):3961-9 PMID: 6193808
  14. Comparison of class I (H-2) gene sequences. Derivation of unique probes for members of this multigene family.
    J Biol Chem. 1983 Nov 25;258(22):13929-36 PMID: 6315713
  15. Variability and conformation of HLA class I antigens: a predictive approach to the spatial arrangement of polymorphic regions.
    Biochemistry. 1984 Feb 28;23(5):823-31 PMID: 6585230
  16. Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3).
    EMBO J. 1984 Apr;3(4):879-85 PMID: 6609813
  17. Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes.
    EMBO J. 1984 Apr;3(4):887-94 PMID: 6609814
  18. Human and murine class I MHC antigens share conserved serine 335, the site of HLA phosphorylation in vivo.
    J Biol Chem. 1984 Jul 25;259(14):9235-40 PMID: 6430898
  19. Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA.
    Immunogenetics. 1984;20(3):237-52 PMID: 6332068
  20. Isolation of HLA locus-specific DNA probes from the 3'-untranslated region.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175-8 PMID: 6591185
  21. Identification of several functional subgroups of HLA-B27 by restriction of the activity of antiviral T killer lymphocytes.
    Immunogenetics. 1984;20(5):513-25 PMID: 6094341
  22. Structure of a functional rabbit class I MHC gene: similarity to human class I genes.
    Immunogenetics. 1985;21(1):71-82 PMID: 3917974
  23. Cloning and complete sequence of an HLA-A2 gene: analysis of two HLA-A alleles at the nucleotide level.
    J Immunol. 1985 Apr;134(4):2727-33 PMID: 2982951
  24. DNA sequence of the mouse H-2Dd transplantation antigen gene.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1175-9 PMID: 3856254
  25. Multiple duplications of complement C4 gene correlate with H-2-controlled testosterone-independent expression of its sex-limited isoform, C4-Slp.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1746-50 PMID: 3856857
  26. An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene.
    Immunogenetics. 1985;21(4):367-83 PMID: 3838967
  27. The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
    Immunogenetics. 1985;21(5):479-89 PMID: 2987115
  28. Primary structure of papain-solubilized human histocompatibility antigen HLA-B27.
    Biochemistry. 1985 Mar 26;24(7):1733-41 PMID: 2408663
  29. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  30. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  31. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 PMID: 6165999
  32. HLA in rheumatology.
    J Rheumatol. 1981 Jul-Aug;8(4):541-6 PMID: 6975375
  33. The age of a neutral mutant persisting in a finite population.
    Genetics. 1973 Sep;75(1):199-212 PMID: 4762875
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.5.1428
Published
1986-03-00
Pages
1428-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323089
Subset
IM
Databases
GENBANK
M12678, M12679
External Links
PubMed source DOI: 10.1073/pnas.83.5.1428 PMC Full Text View journal details
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