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PMID: 3486413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 10 ·1986-05-00 ·Pages 3101-5

Kaushansky K, O'Hara PJ, Berkner K, Segal GM, Hagen FS, Adamson JW

Abstract

Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

MeSH Terms
Base Sequence Cloning, Molecular Colony-Stimulating Factors/genetics,pharmacology Gene Expression Regulation/drug effects Granulocytes Hematopoiesis/drug effects Humans Macrophages Promoter Regions, Genetic Structure-Activity Relationship
Chemicals
Colony-Stimulating Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kaushansky K
O'Hara P J
Berkner K
Segal G M
Hagen F S
Adamson J W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.10.3101
Published
1986-05-00
Pages
3101-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323460
Subset
IM
Grants
NIAID NIH HHS · AI 22772 · United States
NIADDK NIH HHS · AM-01304 · United States
NCI NIH HHS · CA-31615 · United States
Databases
GENBANK
M13207
External Links
PubMed source DOI: 10.1073/pnas.83.10.3101 PMC Full Text View journal details
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