Comparison of in vivo and in vitro multiplication rates of Haemophilus influenzae type b.

Infection and immunity ·Vol. 52 ·No. 3 ·1986-06-00 ·Pages 911-3

Rubin LG

Abstract

A model of invasive Haemophilus influenzae type b disease in rats was used to compare the net in vivo and in vitro multiplication rates of this bacterium. In both the blood of rats and in vitro cultures (fresh rat blood or enriched broth) there was an exponential increase in the number of CFU per milliliter with calculated net mean generation times as follows: sham-operated rats, 82 +/- 39 min (n = 5); asplenic rats, 34 +/- 5 min (n = 13); broth, 26 +/- 4 min (n = 12); rat blood, 24 +/- 1 min (n = 14). Thus, in vivo multiplication of H. influenzae type b can be extremely efficient.

MeSH Terms

Animals Haemophilus Infections/microbiology Haemophilus influenzae/growth & development Rats Splenectomy

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Rubin L G

References (16)

16 references, click to expand

Haemophilus influenzae b infection in rats: effect of splenectomy on bloodstream and meningeal invasion after intravenous and intranasal inoculations.
Infect Immun. 1980 Mar;27(3):872-5 PMID: 6966613
Survival and multiplication of Vibrio cholerae in the upper bowel of infant mice.
Infect Immun. 1978 Nov;22(2):435-40 PMID: 730364
Enhancement of bacterial infection by ferric iron: kinetics, mechanisms, and surgical significance.
Surgery. 1971 Jul;70(1):71-7 PMID: 4933442
The growth of micro-organisms in vivo with particular reference to the relation between dose and latent period.
J Hyg (Lond). 1958 Sep;56(3):323-46 PMID: 13587983
The true division and death rates of Salmonella typhimurium in the mouse spleen determined with superinfecting phage P22.
Br J Exp Pathol. 1968 Dec;49(6):597-613 PMID: 4884387
Inactivation of the bactericidal activity of human serum by liquoid (sodium polyanetholsulfonate).
Appl Microbiol. 1969 Jun;17(6):839-42 PMID: 4894722
Microbial growth rates in nature.
Bacteriol Rev. 1971 Mar;35(1):39-58 PMID: 4929658
The third component of the guinea pig complement system. II. Kinetic study of the reaction of EAC'4,2a with guinea pig C'3. Enzymatic nature of C'3 comsumption, multiphasic character of fixation, and hemolytic titration of C'3.
Biochemistry. 1968 Aug;7(8):2997-3002 PMID: 5666766
Surface determinants of Haemophilus influenzae pathogenicity: comparative virulence of capsular transformants in normal and complement-depleted rats.
J Infect Dis. 1983 Sep;148(3):385-94 PMID: 6604759
Role of intravascular replication in the pathogenesis of experimental bacteremia due to Haemophilus influenzae type b.
J Infect Dis. 1985 Aug;152(2):307-14 PMID: 3875663
The paradox of Hemophilus infuenzae type B bacteremia in the presence of serum bactericidal activity.
J Clin Invest. 1976 Oct;58(4):1019-29 PMID: 1085778
Cellular resistance to infection.
J Exp Med. 1962 Sep 1;116:381-406 PMID: 14467923
Pathogenesis of bloodstream invasion with Haemophilus influenzae type b.
Infect Immun. 1983 Jul;41(1):280-4 PMID: 6602768
Dynamics of bacterial infections in mice under conditions known to alter survival time.
J Infect Dis. 1956 Mar-Apr;98(2):198-207 PMID: 13319779
Quantitative determination of bacterial replication in vivo.
Infect Immun. 1985 Aug;49(2):424-7 PMID: 3926651
Virulence in leptospira. II. The growth in vivo of virulent Leptospira icterohaemorrhagiae.
Br J Exp Pathol. 1957 Feb;38(1):8-14 PMID: 13457343

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.52.3.911-913.1986

Published

1986-06-00

Pages

911-3

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC260949

Subset

External Links

PubMed source DOI: 10.1128/iai.52.3.911-913.1986 PMC Full Text View journal details

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Comparison of in vivo and in vitro multiplication rates of Haemophilus influenzae type b.

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