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PMID: 3487087 Published · ppublish English Journal Article

Change in antigen specificity of cytotoxic T lymphocytes is associated with the rearrangement and expression of a T-cell receptor beta-chain gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 12 ·1986-06-00 ·Pages 4441-5

Epplen JT, Bartels F, Becker A, Nerz G, Prester M, Rinaldy A, Simon MM

Abstract

Cloned H-Y-specific murine cytotoxic T lymphocytes, which alter antigen specificity in vitro ("aging"), simultaneously exhibit changes in the T-cell antigen receptor beta-chain rearrangements and respective mRNAs expressed. beta-chain cDNA clones were isolated from a library prepared from mRNA of aged killer T cells. The sequence of the beta-chain variable region element (VAK) was found to be identical with germ-line DNA. Four bases at the beta-chain diversity-joining region (D beta--J beta) junction cannot be explained by known germ-line D beta and J beta elements. These results illustrate that in T-cell clones altered antigen specificity correlates with a switch in productive beta-chain rearrangements of the T-cell receptor. When tested for its expression under physiological conditions, significant levels of VAK mRNA were found in normal lymphocyte populations.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cytotoxicity, Immunologic DNA/genetics Gene Expression Regulation Mice Receptors, Antigen, T-Cell/genetics Recombination, Genetic T-Lymphocytes, Cytotoxic/immunology
Chemicals
Receptors, Antigen, T-Cell DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Epplen J T
Bartels F
Becker A
Nerz G
Prester M
Rinaldy A
Simon M M
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.12.4441
Published
1986-06-00
Pages
4441-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323749
Subset
IM
Databases
GENBANK
M12775, N00046
External Links
PubMed source DOI: 10.1073/pnas.83.12.4441 PMC Full Text View journal details
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