Specific bioactivities of monocyte-derived interleukin 1 alpha and interleukin 1 beta are similar to each other on cultured murine thymocytes and on cultured human connective tissue cells.

The Journal of clinical investigation ·Vol. 78 ·No. 3 ·1986-09-00 ·Pages 836-9

Rupp EA, Cameron PM, Ranawat CS, Schmidt JA, Bayne EK

Abstract

In this report we compare the bioactivities of pure, human monocyte-derived interleukin 1 (IL-1) alpha and beta in the standard murine thymocyte proliferation assay, a human dermal fibroblast proliferation assay, and in an assay measuring stimulation of prostaglandin E2 (PGE2) release from human rheumatoid synoviocytes. In each case the different species of IL-1 produced saturable stimulation and gave similar dose response curves. Half-maximal stimulation was observed at average IL-1 concentrations of 29 pM in the thymocyte assay, 2 pM in the dermal fibroblast proliferation assay, and 5 pM in the synovial cell assay. Our results show that native, monocyte-derived IL-1 alpha and IL-1 beta are both potent stimulators of connective tissue cells and that the specific bioactivities of these molecules are similar to each other in tests on human connective tissue cells, as well as on murine lymphoid cells.

MeSH Terms

Adult Animals Arthritis, Rheumatoid/metabolism Biological Assay Cell Division Cells, Cultured Dinoprostone Fibroblasts/cytology Humans Interleukin-1/physiology Mice Prostaglandins E/metabolism Synovial Membrane/metabolism Thymus Gland/cytology

Chemicals

Interleukin-1 Prostaglandins E Dinoprostone

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Rupp E A

Cameron P M

Ranawat C S

Schmidt J A

Bayne E K

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25 references, click to expand

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI112649

Published

1986-09-00

Pages

836-9

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC423684

Subset

External Links

PubMed source DOI: 10.1172/JCI112649 PMC Full Text View journal details

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Specific bioactivities of monocyte-derived interleukin 1 alpha and interleukin 1 beta are similar to each other on cultured murine thymocytes and on cultured human connective tissue cells.

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