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PMID: 3494191 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Epidermal growth factor receptor gene-amplified MDA-468 breast cancer cell line and its nonamplified variants.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 251-7

Filmus J, Trent JM, Pollak MN, Buick RN

Abstract

We have recently reported (J. Filmus, M. N. Pollak, R. Cailleau, and R. N. Buick, Biochem. Biophys. Res. Commun. 128:898-905, 1985) that MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited in vitro pharmacological doses of EGF. We have derived several MDA-468 clonal variants which are resistant to EGF-induced growth inhibition. These clones had a number of EGF receptors, similar to normal human fibroblasts, and had lost the EGF receptor gene amplification. Karyotype analysis showed that MDA-468 cells had an abnormally banded region (ABR) in chromosome 7p which was not present in the variants. It was shown by in situ hybridization that the amplified EGF receptor sequences were located in that chromosome, 7pABR. Five of the six variants studied were able to generate tumors in nude mice, but their growth rate was significantly lower than that of tumors derived from the parental cell line. The variant that was unable to produce tumors was found to be uniquely dependent on EGF for growth in soft agar.

MeSH Terms
Breast Neoplasms/genetics,metabolism Cell Division/drug effects Cell Line Chromosomes, Human, Pair 7 DNA, Neoplasm/genetics,isolation & purification Epidermal Growth Factor/pharmacology ErbB Receptors/genetics Female Gene Amplification Genes Genetic Variation Humans Karyotyping Nucleic Acid Hybridization
Chemicals
DNA, Neoplasm Epidermal Growth Factor ErbB Receptors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Filmus J
Trent J M
Pollak M N
Buick R N
References (24)
24 references, click to expand
  1. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies.
    Cancer Res. 1970 Apr;30(4):1174-84 PMID: 5533992
  2. Epidermal growth factor stimulates the synthesis of its own receptor in a human breast cancer cell line.
    J Biol Chem. 1986 Mar 25;261(9):4134-8 PMID: 3005320
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line.
    J Natl Cancer Inst. 1979 Feb;62(2):263-71 PMID: 283262
  5. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  6. Precise localization of human beta-globin gene complex on chromosome 11.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5239-42 PMID: 291941
  7. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  8. Protocols of procedures and techniques in chromosome analysis of tumor stem cell cultures in soft agar.
    Prog Clin Biol Res. 1980;48:345-9 PMID: 6163160
  9. Increased phosphotyrosine content and inhibition of proliferation in EGF-treated A431 cells.
    Nature. 1981 Sep 24;293(5830):305-7 PMID: 6268987
  10. Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7809-13 PMID: 6818550
  11. Biological action of epidermal growth factor and its functional receptors in normal mammary epithelial cells.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2647-50 PMID: 6302699
  12. Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells.
    Science. 1984 Apr 27;224(4647):417-9 PMID: 6200934
  13. Biosynthesis of the epidermal growth factor receptor in A431 cells.
    EMBO J. 1984 Mar;3(3):531-7 PMID: 6325174
  14. Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells.
    Science. 1984 May 25;224(4651):843-8 PMID: 6326261
  15. Relation of epidermal growth factor receptor concentration to growth of human epidermoid carcinoma A431 cells.
    J Biol Chem. 1984 Jun 25;259(12):7761-6 PMID: 6330080
  16. Characterization of epidermal growth factor receptor gene expression in malignant and normal human cell lines.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7308-12 PMID: 6095284
  17. Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin.
    Nature. 1985 Jan 10-18;313(5998):144-7 PMID: 2981413
  18. MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF.
    Biochem Biophys Res Commun. 1985 Apr 30;128(2):898-905 PMID: 2986629
  19. Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells.
    Mol Cell Biol. 1985 Jul;5(7):1722-34 PMID: 2991749
  20. Amplified, overexpressed and rearranged epidermal growth factor receptor gene in a human astrocytoma cell line.
    Biochem Biophys Res Commun. 1985 Aug 30;131(1):207-15 PMID: 2994648
  21. Hyperproduction and gene amplification of the epidermal growth factor receptor in squamous cell carcinomas.
    Jpn J Cancer Res. 1985 Aug;76(8):663-6 PMID: 2995289
  22. Evidence for increased epidermal growth factor receptors in human sarcomas.
    Int J Cancer. 1985 Dec 15;36(6):689-93 PMID: 2999006
  23. Anchorage-independent growth of normal human mesothelial cells: a sensitive bioassay for EGF which discloses the absence of this factor in fetal calf serum.
    In Vitro Cell Dev Biol. 1985 Jan;21(1):67-72 PMID: 3878841
  24. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.251-257.1987
Published
1987-01-00
Pages
251-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365064
Subset
IM
Grants
NCI NIH HHS · CA-12094 · United States
NCI NIH HHS · CA-29476 · United States
NCI NIH HHS · CA-29526 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.1.251-257.1987 PMC Full Text View journal details
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