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PMID: 3500166 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

alpha-Amylase gene of Streptomyces limosus: nucleotide sequence, expression motifs, and amino acid sequence homology to mammalian and invertebrate alpha-amylases.

Journal of bacteriology ·Vol. 169 ·No. 12 ·1987-12-00 ·Pages 5745-54

Long CM, Virolle MJ, Chang SY, Chang S, Bibb MJ

Abstract

The nucleotide sequence of the coding and regulatory regions of the alpha-amylase gene (aml) of Streptomyces limosus was determined. High-resolution S1 mapping was used to locate the 5' end of the transcript and demonstrated that the gene is transcribed from a unique promoter. The predicted amino acid sequence has considerable identity to mammalian and invertebrate alpha-amylases, but not to those of plant, fungal, or eubacterial origin. Consistent with this is the susceptibility of the enzyme to an inhibitor of mammalian alpha-amylases. The amino-terminal sequence of the extracellular enzyme was determined, revealing the presence of a typical signal peptide preceding the mature form of the alpha-amylase.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Codon/genetics DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Protein Biosynthesis Streptomyces/genetics Transcription, Genetic alpha-Amylases/genetics
Chemicals
Codon DNA, Bacterial alpha-Amylases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Long C M
Department of Microbial Genetics, Cetus Corporation, Emeryville, California 94608.
Virolle M J
Chang S Y
Chang S
Bibb M J
References (59)
59 references, click to expand
  1. Aromatic-aromatic interaction: a mechanism of protein structure stabilization.
    Science. 1985 Jul 5;229(4708):23-8 PMID: 3892686
  2. The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis.
    Mol Gen Genet. 1987 Aug;209(1):101-9 PMID: 17165236
  3. The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors.
    Gene. 1985;35(3):223-35 PMID: 2995202
  4. Structure of two divergent promoters located in front of the gene encoding pullulanase in Klebsiella pneumoniae and positively regulated by the malT product.
    J Bacteriol. 1985 Nov;164(2):639-45 PMID: 3902792
  5. The primary structure of alpha-amylase inhibitor Z-2685 from Streptomyces parvullus FH-1641. Sequence homology between inhibitor and alpha-amylase.
    Biol Chem Hoppe Seyler. 1985 Dec;366(12):1161-8 PMID: 3879177
  6. Analysis of signals for secretion in the staphylococcal protein A gene.
    EMBO J. 1985 Dec 30;4(13B):3901-6 PMID: 3912176
  7. Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein.
    J Biol Chem. 1986 Feb 25;261(6):2946-53 PMID: 3005273
  8. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.
    Nucleic Acids Res. 1986 Feb 11;14(3):1319-24 PMID: 3951988
  9. Estimate of the genome size by renaturation studies in Streptomyces.
    Appl Microbiol. 1975 Aug;30(2):324-6 PMID: 1164013
  10. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  11. Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli.
    J Mol Biol. 1978 Sep 15;124(2):359-71 PMID: 101676
  12. Structure-independent nucleotide sequence analysis.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2232-5 PMID: 287063
  13. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. DNA sequence analysis by primed synthesis.
    Methods Enzymol. 1980;65(1):560-80 PMID: 6154869
  16. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  17. Structure of a family of rat amylase genes.
    Nature. 1980 Sep 11;287(5778):117-22 PMID: 6159531
  18. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  19. [The sequence of the alpha-amylase inhibitor Hoe-467 A (alpha-amylase inactivator Hoe-467 A) from Streptomyces tendae 4158].
    Hoppe Seylers Z Physiol Chem. 1981 Apr;362(4):465-7 PMID: 6165665
  20. Expression of the Streptomyces enzyme endoglycosidase H in Escherichia coli.
    J Biol Chem. 1981 Oct 25;256(20):10640-4 PMID: 6270125
  21. Amino acid sequence of hog pancreatic alpha-amylase isoenzyme I.
    FEBS Lett. 1981 Dec 28;136(2):231-4 PMID: 6173261
  22. Los Alamos sequence analysis package for nucleic acids and proteins.
    Nucleic Acids Res. 1982 Jan 11;10(1):183-96 PMID: 6174934
  23. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  24. Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans.
    Mol Gen Genet. 1982;187(2):265-77 PMID: 6294463
  25. Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene.
    J Biol Chem. 1983 Jan 25;258(2):1007-13 PMID: 6185474
  26. Nucleotide sequence of the amylase gene from Bacillus subtilis.
    Nucleic Acids Res. 1983 Jan 25;11(2):237-49 PMID: 6186986
  27. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  28. Isolation and sequence analysis of a barley alpha-amylase cDNA clone.
    J Biol Chem. 1983 Jul 10;258(13):8169-74 PMID: 6190808
  29. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  30. Numerical classification of Streptomyces and related genera.
    J Gen Microbiol. 1983 Jun;129(6):1743-813 PMID: 6631406
  31. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.
    J Gen Microbiol. 1983 Jul;129(7):2257-69 PMID: 6631413
  32. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
    J Gen Microbiol. 1983 Sep;129(9):2703-14 PMID: 6313861
  33. Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
    J Biol Chem. 1984 Feb 10;259(3):1695-702 PMID: 6319407
  34. Graphic methods to determine the function of nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521-38 PMID: 6364040
  35. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  36. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  37. Structure and possible catalytic residues of Taka-amylase A.
    J Biochem. 1984 Mar;95(3):697-702 PMID: 6609921
  38. Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs.
    EMBO J. 1984 May;3(5):1097-102 PMID: 6203744
  39. Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases [corrected].
    Gene. 1984 May;28(2):263-70 PMID: 6610603
  40. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H.
    J Biol Chem. 1984 Jun 25;259(12):7577-83 PMID: 6429133
  41. Tendamistat (HOE 467), a tight-binding alpha-amylase inhibitor from Streptomyces tendae 4158. Isolation, biochemical properties.
    Eur J Biochem. 1984 Jun 15;141(3):505-12 PMID: 6611258
  42. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  43. The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
    Nucleic Acids Res. 1984 Jul 11;12(13):5355-67 PMID: 6087283
  44. Cloning and expression of an extracellular-agarase from Streptomyces coelicolor A3(2) in Streptomyces lividans 66.
    Gene. 1984 Sep;29(3):315-21 PMID: 6489757
  45. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences.
    Gene. 1984 Oct;30(1-3):157-66 PMID: 6096212
  46. Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus.
    J Bacteriol. 1985 Feb;161(2):567-73 PMID: 3918017
  47. Essential and nonessential sequences in malPp, a positively controlled promoter in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1201-8 PMID: 3156124
  48. Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.
    J Bacteriol. 1985 Apr;162(1):102-5 PMID: 3980433
  49. Conserved amino acid sequence domains in alpha-amylases from plants, mammals, and bacteria.
    Biochem Biophys Res Commun. 1985 Apr 16;128(1):470-6 PMID: 3921026
  50. Isolation and structure elucidation of an alpha-amylase inhibitor, AI-3688, from Streptomyces aureofaciens.
    FEBS Lett. 1985 Jun 3;185(1):187-90 PMID: 2581812
  51. Control of lysogeny and immunity of Bacillus subtilis temperate bacteriophage SP beta by its d gene.
    J Bacteriol. 1986 Sep;167(3):952-9 PMID: 3091583
  52. Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator.
    Mol Gen Genet. 1986 Jun;203(3):468-78 PMID: 3018431
  53. Molecular cloning of a xylanase gene from Streptomyces sp. No. 36a and its expression in streptomycetes.
    J Antibiot (Tokyo). 1986 Jul;39(7):985-93 PMID: 3759652
  54. The alpha-amylase gene in Drosophila melanogaster: nucleotide sequence, gene structure and expression motifs.
    Nucleic Acids Res. 1986 Nov 11;14(21):8399-411 PMID: 3024105
  55. Molecular cloning and characterization of the Streptomyces hygroscopicus alpha-amylase gene.
    J Bacteriol. 1987 Mar;169(3):1029-36 PMID: 3029013
  56. Cloning of the xylanase gene of Streptomyces lividans.
    Gene. 1986;49(3):323-9 PMID: 3569919
  57. Engineering for protein secretion in gram-positive bacteria.
    Methods Enzymol. 1987;153:507-16 PMID: 3323808
  58. The agarase gene (dag A) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned gene product.
    J Gen Microbiol. 1987 Aug;133(8):2089-96 PMID: 3443853
  59. Amino acid sequence of protein alpha-amylase inhibitor from Streptomyces griseosporeus YM-25.
    J Biochem. 1985 Apr;97(4):1129-33 PMID: 3875608
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.12.5745-5754.1987
Published
1987-12-00
Pages
5745-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214104
Subset
IM
Databases
GENBANK
M18244
External Links
PubMed source DOI: 10.1128/jb.169.12.5745-5754.1987 PMC Full Text View journal details
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