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PMID: 3510186 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Posttranscriptional autoregulation of Escherichia coli threonyl tRNA synthetase expression in vivo.

Journal of bacteriology ·Vol. 165 ·No. 1 ·1986-01-00 ·Pages 198-203

Butler JS, Springer M, Dondon J, Grunberg-Manago M

Abstract

Five mutations in thrS, the gene for threonyl-tRNA synthetase, have been characterized, and the sites of the mutations have been localized to different regions of the thrS gene by recombination with M13 phage carrying portions of the thrS gene. Quantitative immunoblotting shows that some of these mutations cause the overproduction of structurally altered threonyl-tRNA synthetase in vivo. The amounts of in vivo thrS mRNA as measured by quantitative hybridization are, however, the same as wild-type levels for each mutant. These results demonstrate that the expression of threonyl-tRNA synthetase is autoregulated at the posttranscriptional level in vivo.

MeSH Terms

Amino Acyl-tRNA Synthetases/genetics Chromosome Mapping Escherichia coli/enzymology Gene Expression Regulation Homeostasis Mutation RNA, Messenger/analysis Threonine-tRNA Ligase/analysis,genetics Transcription, Genetic

Chemicals

RNA, Messenger Amino Acyl-tRNA Synthetases Threonine-tRNA Ligase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Butler J S

Springer M

Dondon J

Grunberg-Manago M

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25 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.165.1.198-203.1986

Published

1986-01-00

Pages

198-203

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214389

Subset

External Links

PubMed source DOI: 10.1128/jb.165.1.198-203.1986 PMC Full Text View journal details

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Posttranscriptional autoregulation of Escherichia coli threonyl tRNA synthetase expression in vivo.

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