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PMID: 7050631 Published · ppublish English Journal Article

Transcription units around the gene for E. coli translation initiation factor IF3 (infC).

Molecular & general genetics : MGG ·Vol. 186 ·No. 2 ·1982-00-00 ·Pages 247-52

Springer M, Plumbridge JA, Trudel M, Graffe M, Grunberg-Manago M

Abstract

The coding properties have been analyzed of in vitro constructed lambda recombinant phages carrying E. coli DNA fragments from around the structural gene for translation initiation factor IF3 (infC). This study shows that infC is expressed independently of the promoter of the threonyl-tRNA synthetase (thrS), which is the genes immediately preceding infC. It also shows that the two genes following infC, namely pheS and pheT, which form the phenylalanyl-tRNA synthetase operon, are not expressed from infC's promoter. Thus the four characterized genes of that region that were previously thought to be transcribed in the same direction are now shown to be expressed in vivo as three separate transcription units.

MeSH Terms
DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Genetic Linkage Operon Peptide Initiation Factors/genetics Phenylalanine-tRNA Ligase/genetics Threonine-tRNA Ligase/genetics Transcription, Genetic
Chemicals
DNA, Bacterial Peptide Initiation Factors Phenylalanine-tRNA Ligase Threonine-tRNA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Springer M
Plumbridge J A
Trudel M
Graffe M
Grunberg-Manago M
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331857
Published
1982-00-00
Pages
247-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331857
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