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PMID: 3520569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Secretion of beta-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 12 ·1986-06-00 ·Pages 4180-4

Minsky A, Summers RG, Knowles JR

Abstract

The secretion of beta-lactamase (EC 3.5.2.6) into the periplasm of Escherichia coli has been followed by pulse-chase labeling at 15 degrees C. Though the periplasmic fraction contains only the mature form of the enzyme, the spheroplast fraction contains the completed precursor and a hitherto undocumented processed form. When whole spheroplasts are treated with trypsin, the processed form in this fraction is completely digested. This is in contrast to the native mature enzyme localized in the periplasm, which is trypsin resistant. The beta-lactamase is evidently processed after translocation to a trypsin-sensitive form that is transiently bound to the periplasmic face of the inner membrane. The release of this processed form into the periplasm occurs concomitantly with a conformational change that results in the soluble, catalytically active, trypsin-resistant structure.

MeSH Terms

Cell Compartmentation Cell Membrane/enzymology Escherichia coli/enzymology Protein Conformation Time Factors Trypsin beta-Lactamases/biosynthesis,metabolism

Chemicals

Trypsin beta-Lactamases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Minsky A

Summers R G

Knowles J R

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.12.4180

Published

1986-06-00

Pages

4180-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC323695

Subset

External Links

PubMed source DOI: 10.1073/pnas.83.12.4180 PMC Full Text View journal details

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Secretion of beta-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change.

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