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PMID: 3523438 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Analysis of the nucleotide sequence of the ereB gene encoding the erythromycin esterase type II.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4987-99

Arthur M, Autissier D, Courvalin P

Abstract

We have determined the nucleotide sequence of the ereB gene of plasmid pIP1527 which confers high-level resistance to erythromycin by inactivation in Escherichia coli. The open reading frame of the ereB gene, 1257-bp, was defined by initiation and termination codons and by cloning in vitro. The corresponding protein has a calculated Mr of 48,118 in close agreement with a previous estimation, 51,000, by electrophoresis of minicell extracts in SDS-polyacrylamide gels. The structure of the modified erythromycin was determined by physico-chemical techniques including mass spectrometry, infrared spectrophotometry and 13C nuclear magnetic resonance. The data obtained indicated that like ereA (Ounissi and Courvalin, 1985) ereB encodes an erythromycin esterase. Comparison of the amino acid sequences of the two isozymes did not reveal any statistically significant homology. Analysis of the nucleotide sequence of the ereB gene suggests that this resistance determinant should be exogenous to E. coli.

MeSH Terms

Amino Acid Sequence Base Composition Base Sequence Carboxylic Ester Hydrolases/genetics Cloning, Molecular Codon Drug Resistance, Microbial Erythromycin/metabolism,pharmacology Escherichia coli/genetics Genes Genes, Bacterial Sequence Homology, Nucleic Acid

Chemicals

Codon Erythromycin Carboxylic Ester Hydrolases erythromycin esterase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Arthur M

Autissier D

Courvalin P

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.12.4987

Published

1986-06-25

Pages

4987-99

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311505

Subset

Databases

GENBANK

X03988

External Links

PubMed source DOI: 10.1093/nar/14.12.4987 PMC Full Text View journal details

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Analysis of the nucleotide sequence of the ereB gene encoding the erythromycin esterase type II.

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