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PMID: 3523486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Suppression of tumorigenicity with continued expression of the c-Ha-ras oncogene in EJ bladder carcinoma-human fibroblast hybrid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5209-13

Geiser AG, Der CJ, Marshall CJ, Stanbridge EJ

Abstract

A human tumor cell line (EJ) expressing an activated c-Ha-ras oncogene was fused with a normal human fibroblast cell line. This fusion resulted in hybrids that behaved as transformed cells in culture but failed to form tumors in nude (athymic) mice. After repeated cell passage, two tumorigenic segregants of the hybrids arose in culture. The levels of expression of activated c-Ha-ras mRNA and its protein product, p21, were similar in the EJ cell line, the nontumorigenic hybrids, and the tumorigenic segregants. DNA transfections of the hybrids were performed with activated c-Ha-ras plasmid constructs, and transfectants expressing a 2-fold level of c-Ha-ras relative to the hybrid cells were found to maintain the nontumorigenic phenotype. We suggest that expression of the active c-Ha-ras oncogene is insufficient for the malignant transformation of these human cells.

MeSH Terms
Animals Cell Transformation, Neoplastic Fibroblasts Humans Hybrid Cells/physiology,transplantation Mice Mice, Nude Neoplasm Transplantation Oncogenes Proto-Oncogene Proteins/genetics,physiology Proto-Oncogene Proteins p21(ras) Transfection Urinary Bladder Neoplasms/genetics
Chemicals
Proto-Oncogene Proteins HRAS protein, human Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Geiser A G
Der C J
Marshall C J
Stanbridge E J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5209
Published
1986-07-00
Pages
5209-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323920
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.14.5209 PMC Full Text View journal details
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