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PMID: 3865197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 7810-4

Guerrero I, Villasante A, Corces V, Pellicer A

Abstract

Young mice injected with the carcinogen N-nitroso-N-methylurea develop thymic lymphomas 2-4 months later. We previously have shown that these tumors frequently contain an activated N-ras gene that can transform rodent fibroblasts in vitro. We report here the intron/exon structure of such an activated N-ras gene and the sequence of its four coding exons. A single nucleotide change is responsible for the transforming alteration, a C----A transversion in the first base of codon 61. Through the use of synthetic oligonucleotides as hybridization probes, we show that this tumor lacks the normal allele of the N-ras gene. The implications of this finding for oncogene dominance are discussed.

MeSH Terms
Alleles Animals Base Sequence Brain/physiology Chromosome Mapping Genes, Dominant Lymphoma/chemically induced,genetics Methylnitrosourea Mice Mutation Proto-Oncogenes Thymus Neoplasms/chemically induced,genetics
Chemicals
Methylnitrosourea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guerrero I
Villasante A
Corces V
Pellicer A
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.7810
Published
1985-12-00
Pages
7810-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390859
Subset
IM
Grants
NCI NIH HHS · CA16239 · United States
NCI NIH HHS · CA36327 · United States
NIGMS NIH HHS · GM32036 · United States
Databases
GENBANK
M12121, M12122, M12123, M12124
External Links
PubMed source DOI: 10.1073/pnas.82.23.7810 PMC Full Text View journal details
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