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PMID: 6089200 Published · ppublish English Journal Article

A position 12-activated H-ras oncogene in all HS578T mammary carcinosarcoma cells but not normal mammary cells of the same patient.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5384-8

Kraus MH, Yuasa Y, Aaronson SA

Abstract

Among 21 human mammary tumors analyzed for transforming genes by transfection of NIH/3T3 cells, only DNA of a carcinosarcoma cell line, HS578T, registered as positive. A Harvey (H)-ras oncogene identified in this line was cloned in biologically active form and the activating lesion was identified as a single nucleotide substitution of adenine for guanine in the 12th codon. This results in substitution of aspartic acid for glycine at this position of the p21 coding sequence. Knowledge that this alteration creates a restriction site polymorphism for Msp I/Hpa II in the H-ras protooncogene made it possible to survey for the presence of the activated H-ras allele in normal cells as well as in clonally derived tumor cell lines of the same patient. We demonstrated the presence of unaltered H-ras alleles in normal HS578 cells. In contrast, every clonally derived HS578T tumor cell line analyzed contained the H-ras oncogene possessing the genetic alteration at position 12. These findings establish that activation of this oncogene was the result of a somatic event selected within all HS578T tumor cells.

MeSH Terms
Amino Acid Sequence Base Sequence Breast/analysis Breast Neoplasms/genetics Carcinosarcoma/genetics Cell Line Cell Transformation, Neoplastic Cloning, Molecular DNA Restriction Enzymes Female Humans Oncogenes Transfection
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kraus M H
Yuasa Y
Aaronson S A
References (49)
49 references, click to expand
  1. Chromosome aberrations in cultured cells deprived of single essential amino acids.
    Exp Cell Res. 1969 Jun;55(3):393-409 PMID: 5815057
  2. Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4926-30 PMID: 6308661
  3. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  4. A human cell line from a pleural effusion derived from a breast carcinoma.
    J Natl Cancer Inst. 1973 Nov;51(5):1409-16 PMID: 4357757
  5. Carcinosarcoma of the breast.
    J Pathol. 1974 Feb;112(2):99-105 PMID: 4834054
  6. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.
    Biochem Biophys Res Commun. 1974 Jul 10;59(1):108-16 PMID: 4366956
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. The clonal evolution of tumor cell populations.
    Science. 1976 Oct 1;194(4260):23-8 PMID: 959840
  9. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  10. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines.
    J Natl Cancer Inst. 1977 Jun;58(6):1795-806 PMID: 864756
  11. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
    Cell. 1977 May;11(1):223-32 PMID: 194704
  12. Establishment and characterization of a cell line of human breast carcinoma origin.
    Eur J Cancer. 1979 May;15(5):659-70 PMID: 228940
  13. Ubiquitous, interspersed repeated sequences in mammalian genomes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398-402 PMID: 6246492
  14. The origin of human cancers.
    Nature. 1981 Jan 29;289(5796):353-7 PMID: 6258076
  15. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts.
    Nature. 1981 Mar 19;290(5803):261-4 PMID: 7207618
  16. Transforming activity of human tumor DNAs.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181-4 PMID: 6940134
  17. Three different human tumor cell lines contain different oncogenes.
    Cell. 1981 Aug;25(2):355-61 PMID: 6269741
  18. Activation of related transforming genes in mouse and human mammary carcinomas.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):5185-9 PMID: 6272293
  19. Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene.
    Nature. 1982 Jun 10;297(5866):474-8 PMID: 6283357
  20. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes.
    Nature. 1982 Jul 22;298(5872):343-7 PMID: 6283384
  21. Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2845-9 PMID: 6953433
  22. Stage-specific transforming genes of human and mouse B- and T-lymphocyte neoplasms.
    Cell. 1982 Apr;28(4):873-80 PMID: 6284377
  23. Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637-40 PMID: 6285355
  24. A transforming gene present in human sarcoma cell lines.
    Nature. 1982 Sep 9;299(5879):171-3 PMID: 6287287
  25. Mechanism of activation of a human oncogene.
    Nature. 1982 Nov 11;300(5888):143-9 PMID: 6290897
  26. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  27. Oncogenes in solid human tumours.
    Nature. 1982 Dec 9;300(5892):539-42 PMID: 7144906
  28. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.
    Nature. 1982 Dec 23;300(5894):762-5 PMID: 7177195
  29. Altered gene products are associated with activation of cellular rasK genes in human lung and colon carcinomas.
    Cell. 1983 Jan;32(1):201-8 PMID: 6825168
  30. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  31. Characterization of a human colon/lung carcinoma oncogene.
    Nature. 1983 Mar 3;302(5903):79-81 PMID: 6298638
  32. Three human transforming genes are related to the viral ras oncogenes.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2112-6 PMID: 6572964
  33. Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1.
    Nature. 1983 Jun 2-8;303(5916):396-400 PMID: 6304521
  34. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  35. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations.
    Nature. 1983 Dec 15-21;306(5944):658-61 PMID: 6318112
  36. Activation of N-ras gene in bone marrow cells from a patient with acute myeloblastic leukaemia.
    Nature. 1984 Feb 2-8;307(5950):476-8 PMID: 6582353
  37. Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas.
    Nature. 1984 Feb 16-22;307(5952):658-60 PMID: 6694757
  38. Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient.
    Science. 1984 Feb 17;223(4637):661-4 PMID: 6695174
  39. Somatic activation of rasK gene in a human ovarian carcinoma.
    Science. 1984 Feb 17;223(4637):698-701 PMID: 6695178
  40. Transforming ras genes from human melanoma: a manifestation of tumour heterogeneity?
    Nature. 1984 Mar 1-7;308(5954):69-72 PMID: 6700714
  41. Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours.
    Nature. 1984 May 31-Jun 6;309(5967):464-6 PMID: 6328318
  42. Mechanism of activation of an N-ras oncogene of SW-1271 human lung carcinoma cells.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3670-4 PMID: 6587382
  43. Human-tumor-derived cell lines contain common and different transforming genes.
    Cell. 1981 Dec;27(3 Pt 2):467-76 PMID: 6101201
  44. Cultivation of human breast carcinomas.
    J Natl Cancer Inst. 1958 Dec;21(6):1131-47 PMID: 13611537
  45. Cultivation of normal and malignant human lung tissue. I. The establishment of three adenocarcinoma cell strains.
    Lab Invest. 1962 Aug;11:638-52 PMID: 14490970
  46. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.
    Nature. 1983 Jun 30;303(5920):775-9 PMID: 6866079
  47. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1.
    Nature. 1983 Aug 11-17;304(5926):497-500 PMID: 6308465
  48. Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations.
    Nature. 1983 Aug 11-17;304(5926):507-13 PMID: 6308467
  49. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
    J Virol. 1969 Nov;4(5):549-53 PMID: 4311790
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.17.5384
Published
1984-09-00
Pages
5384-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391708
Subset
IM
Databases
GENBANK
J00206, J00276, J00277, K00954
External Links
PubMed source DOI: 10.1073/pnas.81.17.5384 PMC Full Text View journal details
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