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PMID: 6587382 Published · ppublish English Journal Article

Mechanism of activation of an N-ras oncogene of SW-1271 human lung carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 12 ·1984-06-00 ·Pages 3670-4

Yuasa Y, Gol RA, Chang A, Chiu IM, Reddy EP, Tronick SR, Aaronson SA

Abstract

An N-ras-related transforming gene was detected in the human lung carcinoma cell line SW-1271 and molecularly cloned. The lesion responsible for its acquisition of transforming activity was localized to a single nucleotide transition from A to G in codon 61 of the predicted protein. This lesion in the second exon results in the substitution of arginine for glutamine at this position. These findings, together with previous studies, indicate that the activation of ras oncogenes in human tumors is most commonly due to point mutations at one of two major "hot spots" in the ras coding sequence.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Cell Transformation, Viral Cloning, Molecular Gene Expression Regulation Humans Lung Neoplasms/microbiology Oncogenes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yuasa Y
Gol R A
Chang A
Chiu I M
Reddy E P
Tronick S R
Aaronson S A
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.12.3670
Published
1984-06-00
Pages
3670-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345280
Subset
IM
Databases
GENBANK
K00082, L00040, L00041
External Links
PubMed source DOI: 10.1073/pnas.81.12.3670 PMC Full Text View journal details
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