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PMID: 352797 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi.

Genetics ·Vol. 89 ·No. 2 ·1978-06-00 ·Pages 281-97

Davidson JN, Hanson MR, Bogorad L

Abstract

Five classes of erythromycin-resistant mutants of Chlamydomonas reinhardi have been identified. Each class corresponds to a different genetic locus, three nuclear and two chloroplast. The three nuclear loci appear to be unlinked, while Conde et al. (1975) have shown that the two chloroplast loci are linked, but not allelic. Mutants in each class have a unique pattern of cross-resistance in vivo to other antibiotics (lincomycin, carbomycin, and cleocin) that affect chloroplast protein synthesis. The chloroplast ribosomes from each class have a distinctive erythromycin-binding reaction in vitro.--Haploid and diploid strains containing combinations of different genes affecting the chloroplast ribosome were constructed to probe ribosome structure. New phenotypes were obtained by such combinations, demonstrating interactions between the gene products of a number of loci specifying ribosome components.

MeSH Terms
Chlamydomonas/genetics Crosses, Genetic Drug Resistance, Microbial Erythromycin/pharmacology Genes Genetic Complementation Test Mutation Protein Biosynthesis Ribosomes/metabolism
Chemicals
Erythromycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Davidson J N
Hanson M R
Bogorad L
References (19)
19 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/89.2.281
Published
1978-06-00
Pages
281-97
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1213838
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/89.2.281 PMC Full Text View journal details
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