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PMID: 3532107 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 19 ·1986-10-00 ·Pages 7216-20

Burgess WH, Mehlman T, Marshak DR, Fraser BA, Maciag T

Abstract

Two endothelial cell growth factors (ECGF) have been purified from bovine brain and termed alpha- and beta-ECGF [Burgess, W. H., Mehlman, T., Friesel, R., Johnson, W. V. & Maciag, T. (1985) J. Biol. Chem. 260, 11389-11392]. Amino acid sequence analysis indicates that beta-ECGF represents a 20 amino acid amino-terminal extension of alpha-ECGF and a 14 amino acid amino-terminal extension of acidic fibroblast growth factor. These data indicate that both alpha-ECGF and acidic fibroblast growth factor may be derived from beta-ECGF by posttranslational processing. Analysis of the amino-terminal 14 residues of beta-ECGF by fast-atom-bombardment mass spectrometry established the amino acid sequence of this region and the identity of the blocking group at the amino terminus (acetyl).

MeSH Terms

Amino Acid Sequence Animals Cattle Cyanogen Bromide Endothelial Growth Factors Fibroblast Growth Factors Growth Substances/genetics Mass Spectrometry Peptide Fragments/analysis Protein Precursors Thermolysin Trypsin

Chemicals

Endothelial Growth Factors Growth Substances Peptide Fragments Protein Precursors Fibroblast Growth Factors Trypsin Thermolysin Cyanogen Bromide

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Burgess W H

Mehlman T

Marshak D R

Fraser B A

Maciag T

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.19.7216

Published

1986-10-00

Pages

7216-20

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC386686

Subset

Grants

NIA NIH HHS · AG04807 · United States

NHLBI NIH HHS · HL310765 · United States

External Links

PubMed source DOI: 10.1073/pnas.83.19.7216 PMC Full Text View journal details

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Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor.

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