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PMID: 3536844 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number.

Journal of bacteriology ·Vol. 168 ·No. 2 ·1986-11-00 ·Pages 1010-3

Austin S, Friedman S, Ludtke D

Abstract

The maintenance of plasmid pBR322 is highly unstable in a polA12 strain of Escherichia coli at 29 degrees C due to severely reduced copy number. Under these conditions, introduction of the par (partition) locus of plasmid P1 or the par (sop) region of F into pBR322 stabilizes it. A region with similar activity was detected in the P7 plasmid. The activity of the P1 par locus was dependent on the P1 parA gene product and was sensitive to par-specified incompatibility.

MeSH Terms
DNA Replication DNA, Bacterial/metabolism Escherichia coli/genetics Genes, Bacterial Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Austin S
Friedman S
Ludtke D
References (17)
17 references, click to expand
  1. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1.
    Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44 PMID: 4922634
  2. Two functions of the E protein are key elements in the plasmid F replication control system.
    J Bacteriol. 1985 Dec;164(3):1262-70 PMID: 2999077
  3. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.
    J Bacteriol. 1972 Mar;109(3):971-8 PMID: 4551758
  4. Compatibility properties of P1 and PhiAMP prophages.
    Mol Gen Genet. 1975 Dec 1;141(3):263-7 PMID: 1107819
  5. Replication of prophage P1 during the cell cycle of Escherichia coli.
    Mol Gen Genet. 1977 Mar 28;152(1):71-6 PMID: 325389
  6. Multiple physical differences in the genome structure of functionally related bacteriophages P1 and P7.
    Mol Gen Genet. 1979 Jun 20;173(3):249-61 PMID: 289897
  7. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli.
    Gene. 1982 Jun;18(3):335-41 PMID: 6290337
  8. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  9. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition.
    Cell. 1983 Feb;32(2):351-60 PMID: 6297791
  10. Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition.
    J Mol Biol. 1983 Sep 15;169(2):353-72 PMID: 6312056
  11. Partition of unit-copy miniplasmids to daughter cells. II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts.
    J Mol Biol. 1983 Sep 15;169(2):373-87 PMID: 6225875
  12. Two mini-F-encoded proteins are essential for equipartition.
    Plasmid. 1983 Jul;10(1):73-81 PMID: 6353453
  13. P1 plasmid replication: multiple functions of RepA protein at the origin.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2588-92 PMID: 3857601
  14. A two-stage molecular model for control of mini-F replication.
    Plasmid. 1985 Jan;13(1):59-69 PMID: 2986188
  15. Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA.
    J Bacteriol. 1985 Jul;163(1):349-54 PMID: 3891736
  16. Partition of unit-copy miniplasmids to daughter cells. III. The DNA sequence and functional organization of the P1 partition region.
    J Mol Biol. 1985 Sep 20;185(2):261-72 PMID: 3903163
  17. The number of sex-factors per chromosome in Escherichia coli.
    Biochem J. 1971 Jan;121(1):93-103 PMID: 4940379
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.2.1010-1013.1986
Published
1986-11-00
Pages
1010-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213585
Subset
IM
Grants
NCI NIH HHS · N01-CO-23909 · United States
External Links
PubMed source DOI: 10.1128/jb.168.2.1010-1013.1986 PMC Full Text View journal details
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