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PMID: 2999077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two functions of the E protein are key elements in the plasmid F replication control system.

Journal of bacteriology ·Vol. 164 ·No. 3 ·1985-12-00 ·Pages 1262-70

Rokeach LA, Søgaard-Andersen L, Molin S

Abstract

By using a plasmid carrying a translational fusion between the E gene of the IncFI plasmid F and the lacZ gene, we located the operator of the autogenously regulated E gene to an inverted repeat overlapping the E-gene promoter and showing perfect homology to part of the sequence found in all the direct repeats of two regions exerting an inhibitory effect on F replication, incB and incC. Excess E protein provided in trans to an F plasmid increased the replication frequency of the F plasmid. This stimulatory effect was counteracted by increased dosages of incB or incC. A model is proposed for the replication control system of F in which the key elements are autoregulation of E-gene expression and titration of E protein by incB and incC.

MeSH Terms
Bacterial Proteins/physiology Base Sequence Cloning, Molecular DNA Replication DNA, Bacterial/analysis Escherichia coli/genetics Plasmids Repetitive Sequences, Nucleic Acid
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rokeach L A
Søgaard-Andersen L
Molin S
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50 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.3.1262-1270.1985
Published
1985-12-00
Pages
1262-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219324
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.164.3.1262-1270.1985 PMC Full Text View journal details
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