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PMID: 6325163 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulated expression of a gene important for replication of plasmid F in E. coli.

The EMBO journal ·Vol. 3 ·No. 2 ·1984-02-00 ·Pages 257-62

Søgaard-Andersen L, Rokeach LA, Molin S

Abstract

Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.

MeSH Terms

Bacterial Proteins/biosynthesis,genetics Base Sequence DNA Replication DNA, Bacterial/genetics Escherichia coli/genetics,metabolism F Factor Gene Expression Regulation Operon Repressor Proteins/genetics beta-Galactosidase/biosynthesis

Chemicals

Bacterial Proteins DNA, Bacterial Repressor Proteins beta-Galactosidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Søgaard-Andersen L

Rokeach L A

Molin S

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29 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-02-00

Pages

257-62

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557332

Subset

External Links

PubMed source PMC Full Text View journal details

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Regulated expression of a gene important for replication of plasmid F in E. coli.

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