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PMID: 6759876 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

Molecular & general genetics : MGG ·Vol. 188 ·No. 2 ·1982-00-00 ·Pages 345-52

Gardner RC, Malcolm L, Bergquist PL, Lane HE

Abstract

Cloning of mini-F DNA segments has led to the identification and mapping of a locus, incD, involved in incompatibility reactions with many IncFI plasmids. The cloned incD locus expressed incompatibility with F, R386, and six other IncFI plasmids but not with ColV3-K30 or pHH507 which lack sequence homology with the incD region. A sequence of 360 bp (48.66-49.02 FKB) was found to be sufficient for expression of incD incompatibility. Multicopy vectors containing incD are compatible with each other, but can be displaced by mini-F plasmids deleted for incD. These results indicate that incD-mediated incompatibility reactions require the presence of replication genes to which incD is normally linked. The degree of incompatibility exercised by incD is moderate compared with that of other inc loci in F, suggesting that incD is involved in an aspect of plasmid maintenance, such as partition, different from the functions of the other inc loci.

MeSH Terms

Bacteriocin Plasmids DNA Replication DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genes, Dominant Genes, Regulator Plasmids Replicon

Chemicals

DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gardner R C

Malcolm L

Bergquist P L

Lane H E

References (26)

26 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332699

Published

1982-00-00

Pages

345-52

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332699

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IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

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