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PMID: 3541896 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Glucose-induced accumulation of inositol trisphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer.

The Biochemical journal ·Vol. 237 ·No. 1 ·1986-07-01 ·Pages 259-63

Turk J, Wolf BA, McDaniel ML

Abstract

Anion-exchange h.p.l.c. analysis of [3H]inositol phosphates derived from glucose-stimulated isolated pancreatic islets that had been prelabelled with myo-[3H]inositol revealed that the predominant inositol trisphosphate was the 1,3,4-isomer [Ins(1,3,4)P3]. The 1,4,5-isomer [Ins(1,4,5)P3] was also detectable, as was a more polar inositol phosphate with the chromatographic properties of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. Glucose-induced accumulation of Ins(1,3,4)P3 was augmented by Li+ and occurred after maximal accumulation of Ins(1,4,5)P3. These findings suggest a possible role for Ins(1,3,4)P3 or its probable precursor Ins(1,3,4,5)P4 in stimulus-secretion coupling in pancreatic islets.

MeSH Terms
Animals Chromatography, High Pressure Liquid Glucose/pharmacology In Vitro Techniques Inositol/metabolism Inositol 1,4,5-Trisphosphate Inositol Phosphates/metabolism Islets of Langerhans/drug effects,metabolism Isomerism Male Rats Rats, Inbred Strains Sugar Phosphates/metabolism
Chemicals
Inositol Phosphates Sugar Phosphates Inositol Inositol 1,4,5-Trisphosphate Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Turk J
Wolf B A
McDaniel M L
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2370259
Published
1986-07-01
Pages
259-63
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146973
Subset
IM
Grants
NIADDK NIH HHS · AM07296-06 · United States
NIADDK NIH HHS · AMO6181 · United States
NIADDK NIH HHS · R01 AM34388 · United States
External Links
PubMed source DOI: 10.1042/bj2370259 PMC Full Text View journal details
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