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PMID: 3543401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria.

Journal of virology ·Vol. 61 ·No. 2 ·1987-02-00 ·Pages 633-7

Goh WC, Sodroski JG, Rosen CA, Haseltine WA

Abstract

Human T-lymphotropic virus type III (HTLV-III/LAV or HIV) contains a gene designated art (anti-repressor transactivator). Here, we report the expression of the art gene product in bacteria and show that the 20-kilodalton (kDa) bacterially expressed art protein is recognized by serum of a patient. The bacterially synthesized art protein competed in an immunological reaction with a 20-kDa protein produced in HTLV-III/LAV-infected lymphocytes. Antiserum to a synthetic oligopeptide corresponding to a sequence in the second exon of the art gene also precipitated the 20-kDa protein in HTLV-III/LAV-infected cells. These results demonstrate that the 20-kDa art gene product is expressed in cell lines that produce HTLV-III/LAV virions.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Gene Products, rev Genes Genes, Viral HIV/genetics Viral Proteins/genetics rev Gene Products, Human Immunodeficiency Virus
Chemicals
Bacterial Proteins Gene Products, rev Viral Proteins rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goh W C
Sodroski J G
Rosen C A
Haseltine W A
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.2.633-637.1987
Published
1987-02-00
Pages
633-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253996
Subset
IM
Grants
NCI NIH HHS · CA 07580 · United States
NCI NIH HHS · CA 40658 · United States
NIH HHS · FOD-0757 · United States
Databases
GENBANK
K03455, M15126, M15127
External Links
PubMed source DOI: 10.1128/JVI.61.2.633-637.1987 PMC Full Text View journal details
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