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PMID: 3550383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: the 5S rRNA gene is separated from the 16S and 23S rRNA genes.

Molecular & general genetics : MGG ·Vol. 205 ·No. 3 ·1986-12-00 ·Pages 428-33

Taschke C, Klinkert MQ, Wolters J, Herrmann R

Abstract

In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors lambda EMBL3 and lambda EMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was generated and the organization of the rRNA genes was determined. The results show that the genes for the 16S and 23S rRNAs are closely spaced and occur only once in the genome, whereas the 5S rRNA gene is separated from the other two genes by more than 4 kb.

MeSH Terms
Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Molecular Weight Mycoplasma/genetics Operon Plasmids RNA, Ribosomal/genetics,isolation & purification
Chemicals
RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Taschke C
Klinkert M Q
Wolters J
Herrmann R
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00338078
Published
1986-12-00
Pages
428-33
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00338078
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