Home LiteratureArticle Details
PMID: 6208457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of ribosomal RNA genes in Mycoplasma capricolum.

Molecular & general genetics : MGG ·Vol. 196 ·No. 2 ·1984-00-00 ·Pages 311-6

Sawada M, Muto A, Iwami M, Yamao F, Osawa S

Abstract

DNA segments carrying rRNA genes of Mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, DNA-RNA hybridization and nucleotide sequencing. The M. capricolum genome has two sets of rRNA gene clusters, where the arrangement is in the order of (5')16S-23S-5S(3'). The spacer region between 16S and 23S rDNA is extremely rich in AT and does not carry any tRNA genes.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA, Bacterial/genetics DNA, Ribosomal/genetics Genes, Bacterial Mycoplasma/genetics RNA, Bacterial/genetics RNA, Ribosomal/genetics
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sawada M
Muto A
Iwami M
Yamao F
Osawa S
References (23)
23 references, click to expand
  1. The number of ribosomal RNA genes in Mycoplasma capricolum.
    Mol Gen Genet. 1981;182(3):502-4 PMID: 6272066
  2. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP.
    Biochim Biophys Acta. 1979 Mar 28;562(1):11-31 PMID: 35231
  3. Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.
    Nucleic Acids Res. 1983 Sep 24;11(18):6301-18 PMID: 6312418
  4. Cell biology of the mycoplasmas.
    Bacteriol Rev. 1972 Sep;36(3):263-90 PMID: 4345848
  5. Nucleotide sequence of the kanamycin resistance transposon Tn903.
    J Mol Biol. 1981 Apr 5;147(2):217-26 PMID: 6270337
  6. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2710-4 PMID: 331313
  9. The mycoplasmas.
    Microbiol Rev. 1978 Jun;42(2):414-70 PMID: 353482
  10. The protein composition of Mycoplasma capricolum.
    Mol Gen Genet. 1982;188(1):7-11 PMID: 6960228
  11. Division of mycoplasmas into subgroups.
    J Gen Microbiol. 1970 Oct;63(2):249-63 PMID: 5515110
  12. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.
    Nucleic Acids Res. 1982 Mar 11;10(5):1607-24 PMID: 6280153
  13. The nucleotide sequence of 5S rRNA from Mycoplasma capricolum.
    Nucleic Acids Res. 1981 Oct 24;9(20):5407-10 PMID: 7301591
  14. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  15. Linkage of 5S RNA and 16S+23S RNA genes on the E. coli chromosome.
    Mol Gen Genet. 1977 Jun 24;153(3):337-41 PMID: 331075
  16. Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum.
    Mol Gen Genet. 1984;196(2):317-22 PMID: 6208458
  17. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  18. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli.
    Cell. 1980 Feb;19(2):393-401 PMID: 6153577
  19. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  20. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli.
    Cell. 1977 Aug;11(4):779-93 PMID: 329999
  21. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli.
    Cell. 1976 Feb;7(2):165-77 PMID: 782716
  22. Partial purification of native rRNA and tRNA cistrons from mycoplasma sp. (Kid).
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1282-9 PMID: 5260931
  23. Phylogenetic analysis of the mycoplasmas.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):494-8 PMID: 6928642
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328064
Published
1984-00-00
Pages
311-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X00922
External Links
PubMed source DOI: 10.1007/BF00328064
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2