Home LiteratureArticle Details
PMID: 6280153 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1607-24

Loughney K, Lund E, Dahlberg JE

Abstract

There are at least nine, and probably ten, ribosomal RNA gene sets in the genome of Bacillus subtilis. Each gene set contains sequences complementary to 16S, 23S and 5S rRNAs. We have determined the nucleotide sequences of two DNA fragments which each contain 165 base pairs of the 16S rRNA gene, 191 base pairs of the 23S rRNA gene, and the spacer region between them. The smaller space region is 164 base pairs in length and the larger one includes an additional 180 base pairs. The extra nucleotides could be transcribed in tRNAIIe and tRNA Ala sequences. Evidence is also presented for the existence of a second spacer region which also contains tRNAIIe and tRNA Ala sequences. No other tRNAs appear to be encoded in the spacer regions between the 16S and 23S rRNA genes. Whereas the nucleotide sequences corresponding to the 16S rRNA, 23S rRNA and the spacer tRNAs are very similar to those of E. coli, the sequences between these structural genes are very different.

MeSH Terms
Bacillus subtilis/genetics Bacteriophage lambda/genetics Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Molecular Weight Nucleic Acid Hybridization RNA, Ribosomal/genetics RNA, Transfer/genetics
Chemicals
RNA, Ribosomal RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loughney K
Lund E
Dahlberg J E
References (39)
39 references, click to expand
  1. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  2. An improved method of testing for evolutionary homology.
    J Mol Biol. 1966 Mar;16(1):9-16 PMID: 5917736
  3. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  4. Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli.
    J Mol Biol. 1967 Feb 14;23(3):337-53 PMID: 4291728
  5. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids. II. Comparison of the 5'-terminal nucleotide sequences of ribosomal RNA's from different organisms.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1595-602 PMID: 4296143
  6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  7. Further improvements in the method of testing for evolutionary homology among proteins.
    J Mol Biol. 1970 Apr 14;49(1):1-14 PMID: 5465386
  8. Physical linkage between 5 s, 16 s and 23 s ribosomal RNA genes in Bacillus subtilis.
    J Mol Biol. 1971 Feb 28;56(1):117-27 PMID: 4995655
  9. Minor components in transfer RNA: their characterization, location, and function.
    Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
  10. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.
    J Biol Chem. 1973 Jul 25;248(14):5024-32 PMID: 4577761
  11. Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome.
    J Mol Biol. 1973 Apr 5;75(2):265-79 PMID: 4199565
  12. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  13. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  14. A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNA.
    FEBS Lett. 1975 May 1;53(2):248-52 PMID: 1095416
  15. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  16. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  17. Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis.
    J Biol Chem. 1976 Jun 10;251(11):3480-8 PMID: 179998
  18. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli.
    Cell. 1976 Feb;7(2):165-77 PMID: 782716
  19. Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.
    J Bacteriol. 1977 Jan;129(1):492-500 PMID: 401500
  20. Ribosomal RNA genes in Bacillus subtilis. Evidence for a cotranscription mechanism.
    Biochim Biophys Acta. 1977 Feb 16;474(4):562-77 PMID: 402154
  21. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  22. 3' terminal sequences of 16S rRNA do not explain translational specificity differences between E. coli and B. stearothermophilus ribosomes.
    Nature. 1977 Jun 2;267(5610):462-5 PMID: 327330
  23. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli.
    Cell. 1977 Aug;11(4):779-93 PMID: 329999
  24. Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2710-4 PMID: 331313
  25. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  26. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 PMID: 358189
  27. Sequence of the 16 S-23 s spacer region in two ribosomal RNA operons of Escherichia coli.
    J Biol Chem. 1979 May 10;254(9):3264-71 PMID: 372188
  28. Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes.
    Gene. 1979 Aug;6(4):331-65 PMID: 159206
  29. Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes.
    J Bacteriol. 1979 Oct;140(1):99-105 PMID: 115842
  30. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  31. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli.
    Cell. 1980 Feb;19(2):393-401 PMID: 6153577
  32. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  33. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.
    Nature. 1980 Aug 28;286(5776):908-10 PMID: 6774264
  34. An abundant cytoplasmic 7S RNA is complementary to the dominant interspersed middle repetitive DNA sequence family in the human genome.
    Cell. 1980 Nov;22(1 Pt 1):209-18 PMID: 6159101
  35. Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence.
    Nucleic Acids Res. 1980 May 24;8(10):2275-93 PMID: 6159576
  36. Sequence homologies between Escherichia coli and chloroplast ribosomal DNA as seen by heteroduplex analysis.
    J Mol Biol. 1980 Sep 15;142(2):247-61 PMID: 7003161
  37. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
    Cell. 1981 Jul;25(1):203-13 PMID: 7023695
  38. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  39. Sequence and secondary structure of the colicin fragment of Bacillus stearothermophilus 16S ribosomal RNA.
    Nucleic Acids Res. 1981 Oct 10;9(19):4909-17 PMID: 7312621
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1607
Published
1982-03-11
Pages
1607-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320553
Subset
IM
Grants
NIGMS NIH HHS · GM07215 · United States
Databases
GENBANK
J01551
External Links
PubMed source DOI: 10.1093/nar/10.5.1607 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2