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PMID: 3553205 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sea urchin maternal and embryonic U1 RNAs are spatially segregated in early embryos.

The Journal of cell biology ·Vol. 104 ·No. 5 ·1987-05-00 ·Pages 1133-42

Nash MA, Kozak SE, Angerer LM, Angerer RC, Schatten H, Schatten G, Marzluff WF

Abstract

We have used in situ hybridization and cell fractionation methods to follow the distribution of U1 RNA and immunofluorescence microscopy to follow the distribution of snRNP proteins in oocytes, eggs, and embryos of several sea urchin species. U1 RNA and U1-specific snRNP antigens are concentrated in germinal vesicles of oocytes. Both appear to relocate after oocyte maturation because they are found primarily, if not exclusively, in the cytoplasm of mature unfertilized eggs. This cytoplasmic residence is maintained during early cleavage and U1 RNA is first detectable in nuclei of micromeres at the 16-cell stage. Between morula and gastrula stages the steady-state concentrations of both RNA and antigens gradually increase in nuclei and decrease in cytoplasm. Surprisingly, analysis of the distribution of newly synthesized U1 RNA shows that it does not equilibrate with the maternal pool. Instead new transcripts are confined to nuclei, while cytoplasmic U1 RNAs are of maternal origin. This lack of equilibration and the conversion of maternal U1 RNAs from nuclear species in oocytes to cytoplasmic in embryos suggests that these RNPs (or RNAs) are structurally altered when released to the cytoplasm at oocyte maturation.

MeSH Terms
Animals Cell Division Cell Nucleus/ultrastructure Embryo, Nonmammalian/cytology Female Fluorescent Antibody Technique Nucleic Acid Hybridization RNA, Small Nuclear/analysis,genetics Sea Urchins/cytology,embryology Transcription, Genetic
Chemicals
RNA, Small Nuclear
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nash M A
Kozak S E
Angerer L M
Angerer R C
Schatten H
Schatten G
Marzluff W F
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33 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.104.5.1133
Published
1987-05-00
Pages
1133-42
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114458
Subset
IM
Grants
NIGMS NIH HHS · GM25553 · United States
NIGMS NIH HHS · GM27789 · United States
NICHD NIH HHS · HD12913 · United States
External Links
PubMed source DOI: 10.1083/jcb.104.5.1133 PMC Full Text View journal details
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