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PMID: 2582239 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1143-50

Morris GF, Marzluff WF

Abstract

Nuclei from sea urchin blastula embryos synthesize a variety of small RNAs, one of which has identical mobility with sea urchin U1 RNA. This RNA is synthesized by RNA polymerase II and, in a hybridization-selection experiment, was selected by the cloned sea urchin U1 gene. The U1 RNA was initiated with ATP, but not GTP, in isolated nuclei with beta-S- and gamma-S-ribonucleotide triphosphates as substrates. The U1 RNA containing thiophosphate at the 5' end was not capped but accumulated as an uncapped transcript from which the thiophosphate could be removed with calf intestinal phosphatase.

MeSH Terms
Animals Blastocyst Cell Nucleus/metabolism Cell-Free System RNA/biosynthesis,genetics RNA Processing, Post-Transcriptional RNA, Small Nuclear Sea Urchins/embryology,genetics Transcription, Genetic
Chemicals
RNA, Small Nuclear RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morris G F
Marzluff W F
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39 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1143-1150.1985
Published
1985-05-00
Pages
1143-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366833
Subset
IM
Grants
NIGMS NIH HHS · GM27789 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.5.1143-1150.1985 PMC Full Text View journal details
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