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PMID: 6194507 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of two linked mouse U1b small nuclear RNA genes.

Nucleic acids research ·Vol. 11 ·No. 18 ·1983-09-24 ·Pages 6255-70

Marzluff WF, Brown DT, Lobo S, Wang SS

Abstract

A 6.9 kilobase Eco R1 fragment containing genes for two U1 RNAs has been isolated from a library of mouse DNA. The two genes code for an RNA which is very similar, if not identical, to mouse U1b RNA as judged by S1 nuclease mapping. This RNA is one base longer than the mouse U1a RNA, human U1 RNA, and rat U1 RNA and differs in six nucleotide substitutions from rat U1 RNA. The two genes are five kilobases apart and the U1 RNAs are coded for on opposite strands of the DNA with the 5' ends juxtaposed. The sequences flanking the genes are identical for 700 bases 5' to the gene and at least 80 bases 3' to the gene.

MeSH Terms
Animals Base Composition Base Sequence Cell Nucleus/analysis DNA/analysis,genetics,isolation & purification DNA Restriction Enzymes Deoxyribonuclease EcoRI Endonucleases Genes Genetic Linkage Humans Mice Nucleic Acid Hybridization RNA/genetics,isolation & purification RNA, Small Nuclear Rats Single-Strand Specific DNA and RNA Endonucleases Species Specificity
Chemicals
RNA, Small Nuclear RNA DNA Endonucleases DNA Restriction Enzymes Deoxyribonuclease EcoRI Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marzluff W F
Brown D T
Lobo S
Wang S S
References (35)
35 references, click to expand
  1. Small molecular weight monodisperse nuclear RNA.
    J Mol Biol. 1968 Dec;38(3):289-304 PMID: 5718554
  2. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
    Eur J Biochem. 1973 Aug 1;37(1):31-40 PMID: 4580885
  3. UV shadowing--a new and convenient method for the location of ultraviolet-absorbing species in polyacrylamide gels.
    Anal Biochem. 1974 May;59(1):162-4 PMID: 4407732
  4. Nucleotide sequence of U-2 ribonucleic acid. The sequence of the 5'-terminal oligonucleotide.
    J Biol Chem. 1975 May 25;250(10):3921-8 PMID: 165189
  5. Low molecular weight RNA species from chromatin.
    Biochemistry. 1975 Aug 12;14(16):3715-24 PMID: 1172446
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  8. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  9. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  10. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1047-51 PMID: 98262
  11. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  12. Immunoglobulin heavy chain gene organization in mice: analysis of a myeloma genomic clone containing variable and alpha constant regions.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):857-61 PMID: 106394
  13. Sensitivity of low molecular weight RNA synthesis to UV radiation.
    Nature. 1979 May 3;279(5708):80-1 PMID: 450080
  14. Are snRNPs involved in splicing?
    Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
  15. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  16. Isolation of yeast histone genes H2A and H2B.
    Cell. 1979 Dec;18(4):1261-71 PMID: 519767
  17. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
    Cell. 1980 May;20(1):119-30 PMID: 6446404
  20. Nucleotide sequences of nuclear U1A RNAs from chicken, rat and man.
    Nucleic Acids Res. 1980 Sep 25;8(18):4143-54 PMID: 6159587
  21. Pyrimidine-specific chemical reactions useful for DNA sequencing.
    Nucleic Acids Res. 1980 Oct 24;8(20):4613-9 PMID: 7443522
  22. Structure and expression of a chicken gene coding for U1 RNA.
    Cell. 1981 Mar;23(3):671-80 PMID: 6164492
  23. The conformation of chicken, rat and human U1A RNAs in solution.
    Nucleic Acids Res. 1981 Feb 25;9(4):841-58 PMID: 6164982
  24. Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):810-4 PMID: 6165010
  25. Isolation of two clusters of mouse histone genes.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4078-82 PMID: 6457299
  26. alpha-Fetoprotein and albumin genes are in tandem in the mouse genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4694-8 PMID: 6170978
  27. Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
    Nucleic Acids Res. 1981 Dec 11;9(23):6351-68 PMID: 6172778
  28. Human U1 loci: genes for human U1 RNA have dramatically similar genomic environments.
    Cell. 1982 May;29(1):257-64 PMID: 6179629
  29. Transcription of a gene for human U1 small nuclear RNA.
    Cell. 1982 May;29(1):265-74 PMID: 6179630
  30. SnRNAs, SnRNPs, and RNA processing.
    Annu Rev Biochem. 1982;51:617-54 PMID: 6180681
  31. Sea urchin small nuclear RNA genes are organized in distinct tandemly repeating units.
    Nucleic Acids Res. 1982 Dec 11;10(23):7677-88 PMID: 6185926
  32. Structural analysis of gene loci for rat U1 small nuclear RNA.
    Nucleic Acids Res. 1983 Mar 25;11(6):1791-801 PMID: 6188110
  33. A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
    Biochemistry. 1983 Feb 1;22(3):645-53 PMID: 6188481
  34. Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs.
    J Biol Chem. 1983 Jul 10;258(13):8151-5 PMID: 6190807
  35. Human U1 RNA pseudogenes may be generated by both DNA- and RNA-mediated mechanisms.
    Mol Cell Biol. 1982 Jul;2(7):815-28 PMID: 6085956
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.18.6255
Published
1983-09-24
Pages
6255-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326371
Subset
IM
Grants
NIGMS NIH HHS · GM 27789 · United States
Databases
GENBANK
K03128, X01623, Y00131
External Links
PubMed source DOI: 10.1093/nar/11.18.6255 PMC Full Text View journal details
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