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PMID: 3553613 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that the sigma 1 protein of reovirus serotype 3 is a multimer.

Journal of virology ·Vol. 61 ·No. 6 ·1987-06-00 ·Pages 1834-41

Bassel-Duby R, Nibert ML, Homcy CJ, Fields BN, Sawutz DG

Abstract

In this report, we study the reovirus serotype 3 (strain Dearing) sigma 1 protein obtained from various sources: from Escherichia coli expressing sigma 1 protein, from reovirus-infected mouse L cells, and from purified reovirions. We demonstrate that the sigma 1 protein is a multimer in its undisrupted form and present biochemical evidence suggesting that the multimer is made up of four sigma 1 subunits.

MeSH Terms
Animals Capsid Proteins Escherichia coli/metabolism L Cells/metabolism Mammalian orthoreovirus 3/analysis Mice Protein Conformation Recombinant Proteins/analysis Reoviridae/analysis Viral Proteins/analysis Virion/analysis
Chemicals
Capsid Proteins Recombinant Proteins Viral Proteins sigma 1 protein, reovirus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bassel-Duby R
Nibert M L
Homcy C J
Fields B N
Sawutz D G
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41 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.6.1834-1841.1987
Published
1987-06-00
Pages
1834-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254187
Subset
IM
Grants
NIAID NIH HHS · 2 RO1 AIHL13178 · United States
NINDS NIH HHS · 5 P50 NS16998 · United States
NHLBI NIH HHS · HL-19259 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.6.1834-1841.1987 PMC Full Text View journal details
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