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PMID: 6095208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and sequencing of the reovirus (serotype 3) S1 gene which encodes the viral cell attachment protein sigma 1.

Nucleic acids research ·Vol. 12 ·No. 22 ·1984-11-26 ·Pages 8699-710

Nagata L, Masri SA, Mah DC, Lee PW

Abstract

The complete sequence of the reovirus (serotype 3) S1 gene was obtained by using cloned cDNA derived from the RNA segment. This gene is 1416 nucleotides in length and contains two open reading frames. The first reading frame has a coding capacity of 455 amino acids, sufficient to account for the known S1 product, protein sigma 1 (42,000 MW). It possesses a signal peptide as well as three possible glycosylation sites. No homology could be detected when this gene sequence and the deduced amino acid sequence were compared to published sequences of the corresponding gene of a human rotavirus. The second reading frame (not in phase with the first) starts at the second ATG recently shown to be a functional initiation site. It has a coding capacity of 120 amino acids. Its outstanding feature is the highly basic amino-terminal region, a characteristic apparently shared by a number of DNA binding proteins. It is speculated that this protein, hitherto undetected, may play a role in mediating viral and/or host nucleic acid transcription.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes DNA Transposable Elements Genes Genes, Viral L Cells Mammalian orthoreovirus 3/genetics Mice Plasmids Reoviridae/genetics Viral Proteins/genetics
Chemicals
DNA Transposable Elements Viral Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nagata L
Masri S A
Mah D C
Lee P W
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.22.8699
Published
1984-11-26
Pages
8699-710
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320408
Subset
IM
Databases
GENBANK
X01161
External Links
PubMed source DOI: 10.1093/nar/12.22.8699 PMC Full Text View journal details
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