Home LiteratureArticle Details
PMID: 3558323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Agrobacterium tumefaciens-mediated transformation of the monocot genus Gladiolus: detection of expression of T-DNA-encoded genes.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1745-6

Graves AC, Goldman SL

Abstract

Agrobacterium tumefaciens was capable of directing the transformation of Gladiolus sp., a monocot genus belonging to the family Iridaceae. Only strains capable of transferring T-DNA formed tumors, sections of which could be cultured in phytohormone-free media. Opine synthase activities were also observed in homogenates made from these tumors.

MeSH Terms
DNA, Bacterial/genetics Genes, Bacterial Plant Tumors/microbiology Plants/microbiology Rhizobium/genetics
Chemicals
DNA, Bacterial T-DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Graves A C
Goldman S L
References (10)
10 references, click to expand
  1. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.
    J Mol Appl Genet. 1982;1(6):499-511 PMID: 7153687
  2. A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
    Biochim Biophys Acta. 1978 Dec 8;527(2):497-500 PMID: 31918
  3. DNA from the A6S/2 crown gall tumor contains scrambled Ti-plasmid sequences near its junctions with plant DNA.
    Cell. 1982 Jul;29(3):1005-14 PMID: 7151163
  4. Structure and transcription of the nopaline synthase gene region of T-DNA.
    Nucleic Acids Res. 1983 Jan 25;11(2):369-85 PMID: 6298724
  5. An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis.
    EMBO J. 1984 Dec 20;3(13):3039-41 PMID: 16453585
  6. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  7. Nopaline synthase: transcript mapping and DNA sequence.
    J Mol Appl Genet. 1982;1(6):561-73 PMID: 7153689
  8. Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from agrobacterium to the plant genome.
    Cell. 1984 Sep;38(2):455-62 PMID: 6467373
  9. Plasmid content and tumor initiation complementation by Agrobacterium tumefaciens IIBNV6.
    J Bacteriol. 1977 Dec;132(3):824-31 PMID: 924973
  10. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA.
    J Mol Appl Genet. 1982;1(4):361-70 PMID: 7108407
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.4.1745-1746.1987
Published
1987-04-00
Pages
1745-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212009
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.4.1745-1746.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2