Dissociability of free and peptidyl-tRNA bound ribosomes.

Molecular biology reports ·Vol. 4 ·No. 2 ·1978-06-16 ·Pages 79-81

Surguchov AP, Fominykch ES, Lyzlova LV

Abstract

The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociation of ribosomes either at high salt concentration or in the presence of dissociation factor (DF) from yeast. After dissociation of yeast ribosomes in 0.5 M KCl, peptidyl-tRNA remains bound to the 60 S subunit. Some characteristics of the termination process and release of nascent polypeptides from yeast ribosomes are discussed.

MeSH Terms

Mutation Peptide Chain Termination, Translational Peptides/metabolism Potassium Chloride/administration & dosage RNA, Transfer/metabolism Ribosomes/metabolism,ultrastructure Saccharomyces cerevisiae/metabolism Temperature

Chemicals

Peptides Potassium Chloride RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Surguchov A P

Fominykch E S

Lyzlova L V

References (11)

11 references, click to expand

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Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1007/BF00775964

Published

1978-06-16

Pages

79-81

Language

English

Region

Netherlands

NLM ID

0403234

Subset

External Links

PubMed source DOI: 10.1007/BF00775964 View journal details

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Dissociability of free and peptidyl-tRNA bound ribosomes.

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