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PMID: 3588284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An unusually compact ribosomal DNA repeat in the protozoan Giardia lamblia.

Nucleic acids research ·Vol. 15 ·No. 10 ·1987-05-26 ·Pages 4065-84

Boothroyd JC, Wang A, Campbell DA, Wang CC

Abstract

The ribosomal RNA (rRNA) genes of the protozoan parasite Giardia lamblia have been analyzed with respect to size, composition and copy number. They are found to be remarkable in several respects. First, the rRNAs themselves are the smallest yet reported for any eukaryotic organism. Second, the genes encoding them are found as an exceptionally small tandemly repeated unit of only 5.4 kilobase-pairs. Third, the genes are extraordinarily G:C rich, even in regions which are highly conserved between all other eukaryotic rRNA genes. Finally, by analogy to other organisms, the 5.8S RNA appears to lack about 15 nucleotides from its 3'-end, a region previously thought to be essential for 5.8S RNA function. We also provide the first estimates of the genomic complexity and total G:C content of this important protozoan pathogen.

MeSH Terms
Animals Base Composition Base Sequence DNA, Ribosomal/genetics Genes Giardia/genetics Multigene Family Nucleic Acid Denaturation RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Ribosomal RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boothroyd J C
Wang A
Campbell D A
Wang C C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.10.4065
Published
1987-05-26
Pages
4065-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340832
Subset
IM
Grants
NIAID NIH HHS · AI19391 · United States
NCRR NIH HHS · RR5354 · United States
Databases
GENBANK
X05396, X05397, Y00331
External Links
PubMed source DOI: 10.1093/nar/15.10.4065 PMC Full Text View journal details
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