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PMID: 359749 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antigen-specific helper factor in man.

The Journal of experimental medicine ·Vol. 148 ·No. 4 ·1978-10-01 ·Pages 1032-43

Mudawwar FB, Yunis EJ, Geha RS

Abstract

Supernates of tetanus toxoid (TT) antigen-stimulated human T cells were studied for the presence of an antigen-specific T-cell helper factor (ASF). Supernates were circulated over an immunosorbent column consisting of insolubilized TT antigen. The material which bound to the column was eluted with 3 M NaCNS and was shown to contain a factor which in the presence of TT-induced specific IgG anti-TT antibody synthesis in autologous B cells without causing readily detectable proliferation. ASF activity was partially inhibited by antisera directed against the B-cell alloantigens of the ASF donor. Immunosorbent columns containing such antisera removed ASF activity. Immunosorbent columns containing antisera to human immunoglobulin heavy chain determinants did not remove ASF activity; whereas immunosorbent columns containing rabbit idiotypic antiserum directed against anti-TT antibodies completely removed ASF activity. ASF was destroyed by treatment with proteolytic enzymes; its molecular weight was estimated by Sephadex G-100 gel column chromatography to be between 25,000 and 75,000 daltons.

MeSH Terms
Antibody Formation B-Lymphocytes/immunology Humans Immunoglobulin G/biosynthesis Immunoglobulin Idiotypes Immunosorbent Techniques Lymphocyte Cooperation Lymphokines/isolation & purification Molecular Weight T-Lymphocytes/immunology Tetanus Antitoxin Tetanus Toxoid
Chemicals
Immunoglobulin G Immunoglobulin Idiotypes Lymphokines Tetanus Antitoxin Tetanus Toxoid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mudawwar F B
Yunis E J
Geha R S
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16 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.148.4.1032
Published
1978-10-01
Pages
1032-43
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185019
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.148.4.1032 PMC Full Text View journal details
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