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PMID: 3624204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cytokinin production by Agrobacterium and Pseudomonas spp.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 4242-8

Akiyoshi DE, Regier DA, Gordon MP

Abstract

The production of cytokinins by plant-associated bacteria was examined by radioimmunoassay. Strains producing trans-zeatin were identified in the genera Agrobacterium and Pseudomonas. Agrobacterium tumefaciens strains containing nopaline tumor-inducing plasmids, A. tumefaciens Lippia isolates, and Agrobacterium rhizogenes strains produced trans-zeatin in culture at 0.5 to 44 micrograms/liter. Pseudomonas solanacearum and Pseudomonas syringae pv. savastanoi produced trans-zeatin at levels of up to 1 mg/liter. In vitro cytokinin biosynthetic activity was measured for representative strains and was found to correlate with trans-zeatin production. The genetic locus for trans-zeatin secretion (tzs) was cloned from four strains: A. tumefaciens T37, A. rhizogenes A4, P. solanacearum K60, and P. syringae pv. savastanoi 1006. Southern blot analysis showed substantial homology of the Agrobacterium tzs genes to each other but not to the two Pseudomonas genes.

MeSH Terms
Cytokinins/biosynthesis DNA, Bacterial/analysis Genes, Bacterial Nucleic Acid Hybridization Plant Growth Regulators/biosynthesis Pseudomonas/genetics,metabolism Purines/biosynthesis Radioimmunoassay Rhizobium/genetics,metabolism Sequence Homology, Nucleic Acid Zeatin/biosynthesis,genetics
Chemicals
Cytokinins DNA, Bacterial Plant Growth Regulators Purines Zeatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Akiyoshi D E
Regier D A
Gordon M P
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17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.9.4242-4248.1987
Published
1987-09-00
Pages
4242-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213736
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.9.4242-4248.1987 PMC Full Text View journal details
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