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PMID: 3714473 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence and organization of genes encoding the human 27 kDa heat shock protein.

Nucleic acids research ·Vol. 14 ·No. 10 ·1986-05-27 ·Pages 4127-45

Hickey E, Brandon SE, Potter R, Stein G, Stein J, Weber LA

Abstract

The 27 kDa human heat shock protein (hsp27) is encoded by a gene family of 4 members. Two genomic fragments hybridizing to cDNA encoding hsp27 have been isolated, characterized, and sequenced. One clone is a member of a cluster of three genes linked within a 14-18 kb region of the genome and encodes a transcript interrupted by two intervening sequences. A single open reading frame encodes a polypeptide of 22,300 deduced molecular weight. The 5' flanking region contains two transcription start sites and sequences homologous to the Drosophila consensus heat inducible control element. Induction of both potential transcripts follows heat shock in vivo. Accurate heat inducible transcription occurs at both start sites after injection into Xenopus oocytes. The second genomic clone is a processed pseudogene lacking promoter elements and is unlinked with the other members of the hsp27 gene family. The amino acid sequence of human hsp27 shows striking homology with mammalian alpha crystallin, and contains a region towards the carboxy terminus which shares homology with the small hsp of Drosophila and other organisms.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Crystallins/genetics Heat-Shock Proteins/genetics Humans Molecular Weight Nucleic Acid Hybridization Transcription, Genetic Xenopus
Chemicals
Crystallins Heat-Shock Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hickey E
Brandon S E
Potter R
Stein G
Stein J
Weber L A
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.10.4127
Published
1986-05-27
Pages
4127-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339850
Subset
IM
Grants
NIGMS NIH HHS · GM32381 · United States
Databases
GENBANK
X03900, X03901
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/nar/14.10.4127 PMC Full Text View journal details
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