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PMID: 37237 Published · ppublish English Journal Article

Effect of cyclic guanosine 3',5'-monophosphate on nitrogen fixation in Rhizobium japonicum.

Journal of bacteriology ·Vol. 139 ·No. 1 ·1979-07-00 ·Pages 256-63

Lim ST, Hennecke H, Scott DB

Abstract

The addition of exogenous cyclic guanosine 3',5'-monophosphate (cGMP) at a concentration of 0.1 mM to a free-living culture of Rhizobium japonicum 3I1b110 was found to completely inhibit the expression of nitrogenase activity and markedly inhibit the expression of hydrogenase and nitrate reductase activities. The effect was specific for cGMP. Experiments on the in vivo incorporation of radioactive methionine and subsequent analysis of the labeled proteins on polyacrylamide gels showed that the biosynthesis of nitrogenase polypeptides was inhibited. It appears that the time of addition of cGMP is important since the effect was only seen during the early stages of nif gene expression. The intracellular level of cGMP was found to respond to physiological changes in the cell, and there was a fall in cGMP concentrations when nitrogenase was induced. Microaerophilic-aerobic shift experiments showed that intracellular levels increased from 0.25 pmol/mg of cell protein under microaerophilic conditions to 2.6 pmol/mg of cell protein under aerobic conditions, suggesting that the cellular pool size of cGMP may be under redox control.

MeSH Terms
Cyclic GMP/metabolism,pharmacology Glutamate-Ammonia Ligase/metabolism Hydrogen Malate Dehydrogenase/metabolism Nitrate Reductases/metabolism Nitrogen Fixation/drug effects Nitrogenase/metabolism Oxidoreductases/metabolism Rhizobium/metabolism
Chemicals
Hydrogen Oxidoreductases Malate Dehydrogenase Nitrogenase Nitrate Reductases Glutamate-Ammonia Ligase Cyclic GMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lim S T
Hennecke H
Scott D B
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.1.256-263.1979
Published
1979-07-00
Pages
256-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216853
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.1.256-263.1979 PMC Full Text View journal details
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