Analysis of rpsD mutations in Escherichia coli. III. Effects of rpsD mutations on expression of some ribosomal protein genes.

Molecular & general genetics : MGG ·Vol. 169 ·No. 3 ·1979-02-01 ·Pages 271-8

Olsson MO, Isaksson LA

Abstract

Relative rates of production and steady state levels of ribosomal proteins were determined in a temperature sensitive rpsD (S4) mutant of Escherichia coli. Some proteins (S4, S12, S13) were overproduced in the mutant at permissive temperature but steady state levels of all examined ribosomal proteins were normal. In a rpsD+/rpsD+ homodiploid strain the relative rates of production of ribosomal proteins were not affected by the increased gene dose. In a rpsD+/rpsD heterodiploid strain only wild type, but not mutant S4, was found. In such a strain S4, S7, S12 and probably S13 is overproduced. It is implied that S4 is involved in the regulation of expression of proximal genes of the two transcriptional units including the genes coding for S4 itself and S12, respectively. A degradation system for ribosomal proteins, which is rapid enough to be of regulatory significance, is demonstrated.

MeSH Terms

Bacterial Proteins/biosynthesis,genetics Escherichia coli/genetics Genes Hot Temperature Mutation Phenotype Ribosomal Proteins/biosynthesis,genetics

Chemicals

Bacterial Proteins Ribosomal Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Olsson M O

Isaksson L A

References (25)

25 references, click to expand

Ribosomal protein neighborhoods. 3. Cooperativity of assembly.
Mol Gen Genet. 1974 Mar 14;129(2):167-76 PMID: 4208880
The requirements for specific sRNA binding by ribosomes.
J Mol Biol. 1966 Jun;18(1):90-108 PMID: 5337561
Synthesis and turnover of basal level guanosine tetraphosphate in Escherichia coli.
J Biol Chem. 1975 Jan 10;250(1):304-9 PMID: 1095568
Synthesis of ribosomal proteins in merodiploid strains and in minicells of Escherichia coli.
Mol Gen Genet. 1977 Sep 9;154(3):327-34 PMID: 337111
Syntheses of elongation factors Tu and G are under stringent control in Escherichia coli.
J Biol Chem. 1976 Feb 10;251(3):898-901 PMID: 765336
Association of nascent ribosomal ribonucleic acid with polyribosomes in Escherichia coli.
J Bacteriol. 1975 Dec;124(3):1330-43 PMID: 1104582
Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1526-30 PMID: 4556463
Genetics of bacterial ribosomes.
Annu Rev Genet. 1977;11:297-347 PMID: 339818
A genetic locus for the regulation of ribonucleic acid synthesis.
Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005-14 PMID: 13916843
Analysis of rpsD mutations in Escherichia coli. I. Comparison of mutants with various alterations in ribosomal protein S4.
Mol Gen Genet. 1979 Feb 1;169(3):251-7 PMID: 372747
Restriction, de-restriction and mistranslation in missense suppression. Ribosomal discrimination of transfer RNA's.
J Mol Biol. 1972 Feb 28;64(1):119-34 PMID: 4552481
Stringent control of the transcriptional activities of ribosomal protein genes in E. coli.
Nature. 1975 Jun 5;255(5508):460-5 PMID: 1094295
Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates.
J Mol Biol. 1977 Sep 25;115(3):335-54 PMID: 338910
Analysis of rpsD mutations in Escherichia coli. II. Physiology of some representative mutants.
Mol Gen Genet. 1979 Feb 1;169(3):259-69 PMID: 372748
Letter: Unequal contribution to ribosomal assembly of both str alleles in Escherichia coli merodiploids and its relationship to the dominance phenomenon.
J Mol Biol. 1974 Jan 15;82(2):273-7 PMID: 4131579
DNA-dependent in vitro synthesis of fibosomal proteins, protein elongation factors, and RNA polymerase subunit alpha: inhibition by ppGpp.
Cell. 1976 Nov;9(3):439-48 PMID: 991274
Ribosomal protein in E. coli: rate of synthesis and pool size at different growth rates.
Mol Gen Genet. 1974 Mar 6;129(1):61-75 PMID: 4600015
The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
On the regulation of guanosine tetraphosphate levels in stringent and relaxed strains of Escherichia coli.
J Biol Chem. 1971 Jul 25;246(14):4381-5 PMID: 4937124
Some effects of antibiotics on bacterial polyribosomes as studied by gel electrophoresis.
J Mol Biol. 1973 Aug 25;78(4):627-36 PMID: 4271657
Pools of ribosomal proteins in Escherichia coli. Studies on the exchange of proteins between pools and ribosomes.
Eur J Biochem. 1975 Sep 1;57(1):49-54 PMID: 1100403
Stringent control of ribosomal protein gene expression in Escherichia coli.
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3819-23 PMID: 4610562
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
Genetic studies of the ribosomal proteins in Escherichia coli. XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains.
Mol Gen Genet. 1978 Apr 6;160(2):151-5 PMID: 349351
Regulation of ribosomal and transfer RNA synthesis.
J Mol Biol. 1962 Mar;4:193-210 PMID: 14460744

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00382273

Published

1979-02-01

Pages

271-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00382273

Analysis Services

Analysis of rpsD mutations in Escherichia coli. III. Effects of rpsD mutations on expression of some ribosomal protein genes.

Contact

WeChat Official Account