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PMID: 3729351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.

Antimicrobial agents and chemotherapy ·Vol. 29 ·No. 5 ·1986-05-00 ·Pages 930-2

Dickgiesser N, Kreiswirth BN

Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

MeSH Terms
Aminoglycosides/pharmacology Anti-Bacterial Agents/pharmacology DNA, Bacterial/analysis Drug Resistance, Microbial Nucleic Acid Hybridization Staphylococcus aureus/drug effects
Chemicals
Aminoglycosides Anti-Bacterial Agents DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dickgiesser N
Kreiswirth B N
References (20)
20 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.29.5.930
Published
1986-05-00
Pages
930-2
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284184
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.29.5.930 PMC Full Text View journal details
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