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PMID: 6271046 Published · ppublish English Journal Article

Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 19 ·No. 5 ·1981-05-00 ·Pages 766-72

Kayser FH, Homberger F, Devaud M

Abstract

A genetic analysis of aminocyclitol resistance in two strains of Staphylococcus aureus was performed. Resistance of strain FK170, isolated in Zurich, was due to the production of a 3'-phosphotransferase [APH(3')]. Strain 5532, isolated in London, produced a 2"-phosphotransferase [APH(2")] and a 6'-N-acetyltransferase [AAC(6')]. Plasmid deoxyribonucleic acid (DNA) was isolated by isopycnic centrifugation from the two parent strains, as well as from susceptible variants and from resistant transductants of both strains. Comparative analysis of plasmid and from resistant transductants of both strains. Comparative analysis of plasmid DNA by centrifugation in sucrose gradients revealed that strain FK170 harbored a 2.7-megadalton tetracycline R-plasmid and a 36-megadalton cryptic plasmid. Strain 5532 contained an 18.5-megadalton penicillinase plasmid. No evidence for plasmid location of the markers for aminocyclitol resistance could be obtained.

MeSH Terms
Acetyltransferases/genetics,metabolism Anti-Bacterial Agents/pharmacology Chromosomes, Bacterial DNA, Bacterial/analysis DNA, Circular/analysis Genes, Bacterial Kanamycin Kinase Phosphotransferases/genetics,metabolism R Factors Staphylococcus aureus/analysis,drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA, Bacterial DNA, Circular Acetyltransferases Phosphotransferases Kanamycin Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kayser F H
Homberger F
Devaud M
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30 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.19.5.766
Published
1981-05-00
Pages
766-72
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC181519
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.19.5.766 PMC Full Text View journal details
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