New plasmid-mediated nucleotidylation of aminoglycoside antibiotics in Staphlococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 10 ·No. 2 ·1976-08-00 ·Pages 258-64

Le Goffic F, Martel A, Capmau ML, Baca B, Goebel P, Chardon H, Soussy CJ, Duval J, Bouanchaud DH

Abstract

A wild-type strain of Staphylococcus aureus, which inactivates a wide variety of aminoglycosides (except the gentamicin components), has been found to harbor a plasmid (RAp01) that mediates the biosynthesis of a nucleotidyltransferase. This enzyme modifies the 4'-hydroxy function of these antibiotics. The plasmid has been studied, the enzyme responsible for this resistance pattern has been isolated by affinity chromatography, and its kinetics and physicochemistry have been characterized. The target of this enzyme has also been located by demonstrating the structure of one inactivated compound, 4'-(O)-adenylyltobramycin.

MeSH Terms

Aminoglycosides/metabolism,pharmacology Anti-Bacterial Agents/metabolism,pharmacology DNA, Bacterial/isolation & purification Extrachromosomal Inheritance Nucleotides/metabolism Plasmids Staphylococcus aureus/drug effects,enzymology,metabolism

Chemicals

Aminoglycosides Anti-Bacterial Agents DNA, Bacterial Nucleotides

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Le Goffic F

Martel A

Capmau M L

Baca B

Goebel P

Chardon H

Soussy C J

Duval J

Bouanchaud D H

References (13)

13 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.10.2.258

Published

1976-08-00

Pages

258-64

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC429732

Subset

External Links

PubMed source DOI: 10.1128/AAC.10.2.258 PMC Full Text View journal details

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New plasmid-mediated nucleotidylation of aminoglycoside antibiotics in Staphlococcus aureus.

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