Lividomycin resistance in staphylococci by enzymatic phosphorylation.
Enzymatic inactivation of lividomycin (LV) was attempted with nine clinical isolates of staphylococci including LV-susceptible and -resistant strains. LV inactivation and the incorporation into LV of (32)P from gamma-(32)P-adenosine triphosphate were demonstrated in the presence of cell-free extracts from LV-resistant strains but not from LV-susceptible ones. The enzyme was purified approximately 82-fold from a resistant Staphylococcus aureus strain by means of ammonium sulfate fractionation and column chromatography. Some properties of the partially purified LV-phosphorylating enzyme were quite similar to those of an enzyme from Escherichia coli carrying an R factor conferring LV resistance, and the phosphorylated product of the drug was also found to be identical with that produced by E. coli carrying an R factor, i.e., 5''-phosphoryl-LV.
19 references, click to expand
-
Phosphorylative inactivation of aminoglycosidic antibiotics by Escherichia coli carrying R factor.Science. 1967 Sep 29;157(3796):1559-61 PMID: 4166859
-
Enzymatic inactivation of streptomycin by R factor-resistant Escherichia coli.Nature. 1968 Jul 20;219(5151):288-91 PMID: 4299554
-
Inactivation and phosphorylation of kanamycin by drug-resistant Staphylococcus aureus.Appl Microbiol. 1968 Sep;16(9):1282-4 PMID: 5676403
-
Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors.J Bacteriol. 1969 Nov;100(2):1144-6 PMID: 4902391
-
Phosphorylated inactivation of aminoglycosidic antibiotics by Pseudomonas aeruginosa.Jpn J Microbiol. 1971 May;15(3):265-72 PMID: 4107282
-
Inactivation of dihydrostreptomycin by Pseudomonas aeruginosa.Jpn J Microbiol. 1971 Jul;15(4):381-2 PMID: 4107311
-
Inactivation of dihydrostreptomycin by Staphylococcus aureus.Jpn J Microbiol. 1971 Nov;15(6):545-8 PMID: 4110812
-
Studies on adenylylstreptomycin, a product of streptomycin inactivation by E. coli carrying the R-factor.J Antibiot (Tokyo). 1968 Aug;21(8):477-84 PMID: 4303538
-
Transmissible multiple drug resistance in Enterobacteriaceae.Science. 1972 May 19;176(4036):758-68 PMID: 5031472
-
Studies on new antibiotic lividomycins. V. In vitro and in vivo antimicrobial activity of lividomycin A.J Antibiot (Tokyo). 1972 Feb;25(2):128-36 PMID: 4624613
-
Isolation of the rec mutants in Staphylococcus aureus.J Bacteriol. 1972 Dec;112(3):1169-76 PMID: 4264448
-
Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor.J Antibiot (Tokyo). 1972 Aug;25(8):483-4 PMID: 4630725
-
Activity of lividomycin against Pseudomonas aeruginosa: its inactivation by phosphorylation induced by resistant strains.Antimicrob Agents Chemother. 1972 Jan;1(1):17-21 PMID: 4207755
-
Phosphorylation of lividomycin by Escherichia coli carrying an R factor.Antimicrob Agents Chemother. 1972 Sep;2(3):142-6 PMID: 4597704
-
Antibacterial activity of lividomycin toward R factor-resistant strains of Escherichia coli.Antimicrob Agents Chemother. 1972 Feb;1(2):139-42 PMID: 4618453
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS.J Gen Microbiol. 1963 Oct;33:121-36 PMID: 14072829
-
ELIMINATION OF PENICILLIN RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE.J Bacteriol. 1964 Jul;88:261-2 PMID: 14197897
-
ELIMINATION OF DRUG RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE.J Bacteriol. 1963 Jul;86:162-4 PMID: 14051810
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