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PMID: 3737413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides.

Nucleic acids research ·Vol. 14 ·No. 14 ·1986-07-25 ·Pages 5605-14

Cornelissen AW, Verspieren MP, Toulmé JJ, Swinkels BW, Borst P

Abstract

Several mature mRNAs of Trypanosoma brucei were previously shown to have a common 5' terminal sequence of 35 nucleotides (nt) encoded by a separate mini-exon. To verify whether all trypanosome mRNAs contain this mini-exon sequence at their 5' end, we have tested oligodeoxynucleotides complementary to different parts of the 35 nt leader sequence for their ability to inhibit translation of total trypanosome mRNA. All oligomers tested inhibited translation of trypanosome mRNAs in a wheat germ extract. They had no effect on translation of Brome mosaic virus mRNA and of a trypanosome mRNA for phosphoglycerate kinase modified to remove the mini-exon sequence. Three different 12mers inhibited translation 35-60%; both the 22- and 34mer inhibited translation 95-100%. Incorporation of amino acids decreased proportionally in all protein bands detected in high resolution polyacrylamide gels. Our results show that all trypanosome mRNAs that yield a product detectable in gel contain a mini-exon sequence. We infer that most, if not all, trypanosome mRNAs contain a 5' terminal mini-exon sequence acquired by discontinuous synthesis.

MeSH Terms
Animals Base Sequence Genes Kinetics Oligodeoxyribonucleotides Phosphoglycerate Kinase/genetics Plants/metabolism Plasmids Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic Triticum/metabolism Trypanosoma brucei brucei/enzymology,genetics
Chemicals
Oligodeoxyribonucleotides RNA, Messenger Phosphoglycerate Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cornelissen A W
Verspieren M P
Toulmé J J
Swinkels B W
Borst P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.14.5605
Published
1986-07-25
Pages
5605-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311579
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.14.5605 PMC Full Text View journal details
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