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PMID: 374345 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enzymatic degradation of uracil-containing deoxyribonucleic acid. V. Survival of Escherichia coli and coliphages treated with sodium bisulfite.

Journal of bacteriology ·Vol. 137 ·No. 3 ·1979-03-00 ·Pages 1243-52

Simmons RR, Friedberg EC

Abstract

A number of mutants of Escherichia coli defective in the ung gene (structural gene for uracil-deoxyribonucleic acid [ura-DNA] glycosylase) are shown to be abnormally sensitive to treatment with sodium bisulfite when compared with congenic ung+ strains. These results provide further evidence that sodium bisulfite causes the deamination of cytosine to uracil in DNA and that ura-DNA glycosylase is required for the repair of U-G mispairs. The effect of the chemical is apparently selective with respect to base damage; coliphages containing cytosine in their DNA are inactivated by treatment with sodium bisulfite, whereas those containing hydroxymethylcytosine are not. ura-DNA glycosylase and the major apurinic-apyrimidinic endonuclease of E. coli may function in the same repair pathway, since the extent of inactivation of a congenic set of strains which are ung xth (structural gene for the major apurinic-apyrimidinic endonuclease of E. coli) or ung xth+ is the same.

MeSH Terms
Biodegradation, Environmental Coliphages/drug effects DNA Repair DNA, Bacterial/metabolism Deoxyuracil Nucleotides/metabolism Escherichia coli/drug effects,genetics,metabolism Mutation N-Glycosyl Hydrolases/genetics,metabolism Sulfites/pharmacology
Chemicals
DNA, Bacterial Deoxyuracil Nucleotides Sulfites N-Glycosyl Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simmons R R
Friedberg E C
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.137.3.1243-1252.1979
Published
1979-03-00
Pages
1243-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218307
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.137.3.1243-1252.1979 PMC Full Text View journal details
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