Home LiteratureArticle Details

PMID: 3743551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.

The EMBO journal ·Vol. 5 ·No. 7 ·1986-07-00 ·Pages 1697-703

Krupp G, Cherayil B, Frendewey D, Nishikawa S, Söll D

Abstract

RNase P activity from Schizosaccharomyces pombe co-purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments. The two RNAs, K1- and K2-RNA, are 285 and 270 nucleotides long, respectively. Both RNAs are transcribed from one gene, present in a single copy in the haploid genome. The primary and a secondary structure of K RNAs have been determined and compared with M1 RNA, their counterpart from Escherichia coli. Very limited sequence homology was observed, and this agrees with the finding that no cross-hybridization with M1 RNA can be detected in a Southern analysis with yeast genomic DNA. However, the secondary structures of K RNA and M1 RNA show the same basic organization and one conserved local motif, the sequence GUG--AGGPu in an exposed hairpin loop.

MeSH Terms

Base Sequence Cloning, Molecular Endoribonucleases/genetics,isolation & purification Escherichia coli Proteins Genes Genes, Fungal Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Fungal/genetics,isolation & purification Ribonuclease P Saccharomycetales/genetics Schizosaccharomyces/enzymology,genetics Transcription, Genetic

Chemicals

Escherichia coli Proteins RNA, Fungal Endoribonucleases Ribonuclease P ribonuclease P, E coli

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Krupp G

Cherayil B

Frendewey D

Nishikawa S

Söll D

References (44)

44 references, click to expand

The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
Science. 1985 Dec 20;230(4732):1344-9 PMID: 2933810
Site specific enzymatic cleavage of RNA.
Nucleic Acids Res. 1979 Sep 11;7(1):179-92 PMID: 386279
Reconstitution of RNase P activity from inactive RNA and protein.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3795-9 PMID: 386349
Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli.
Biochemistry. 1986 Apr 8;25(7):1509-15 PMID: 2423112
Generalized structures of the 5S ribosomal RNAs.
Nucleic Acids Res. 1982 Nov 25;10(22):7323-44 PMID: 7155895
Comparison of the structures of carboxypeptidase A and thermolysin.
J Biol Chem. 1977 Nov 10;252(21):7704-10 PMID: 914833
Structure in solution of M1 RNA, the catalytic subunit of ribonuclease P from Escherichia coli.
Biochemistry. 1984 Dec 18;23(26):6327-34 PMID: 6085007
Compilation of tRNA sequences.
Nucleic Acids Res. 1985;13 Suppl:r1-49 PMID: 3846903
Secondary structure and evolution of ribosomal RNA.
Nature. 1981 Nov 19;294(5838):209-10 PMID: 7300908
A plasmid cloning vehicle allowing a positive selection for inserted fragments.
Gene. 1980 Dec;12(1-2):123-7 PMID: 6260582
Preparation of RNA and ribosomes from yeast.
Methods Cell Biol. 1975;12:45-64 PMID: 1105073
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.
Cell. 1983 Dec;35(3 Pt 2):849-57 PMID: 6197186
First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes.
Mol Gen Genet. 1985;201(1):82-7 PMID: 3903436
The conserved 5 S rRNA complement to tRNA is not required for translation of natural mRNA.
J Biol Chem. 1984 Mar 10;259(5):2798-802 PMID: 6365917
Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases.
Gene. 1984 Nov;31(1-3):129-34 PMID: 6526270
Isolation, characterization, and translation of mRNA from yeast.
Methods Cell Biol. 1978;20:61-81 PMID: 357927
Processing of tRNA in prokaryotes and eukaryotes.
CRC Crit Rev Biochem. 1984;17(1):45-71 PMID: 6094100
Hybrid selection of specific RNAs using DNA covalently coupled to macroporous supports.
Methods Enzymol. 1983;100:400-7 PMID: 6194409
Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562-6 PMID: 3001709
Structure and transcription of eukaryotic tRNA genes.
CRC Crit Rev Biochem. 1985;19(2):107-44 PMID: 3905254
Ribosomal RNA synthesis in Saccharomyces cerevisiae.
J Mol Biol. 1972 Mar 28;65(2):227-42 PMID: 4557192
Halobacterium volcanii tRNAs. Identification of 41 tRNAs covering all amino acids, and the sequences of 33 class I tRNAs.
J Biol Chem. 1984 Aug 10;259(15):9461-71 PMID: 6746655
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500 PMID: 6757864
Estimation of secondary structure in ribonucleic acids.
Nature. 1971 Apr 9;230(5293):362-7 PMID: 4927725
Partial purification of RNase P from Schizosaccharomyces pombe.
J Biol Chem. 1981 May 25;256(10):5058-63 PMID: 6262315
Small nuclear RNAs in the ciliate Tetrahymena.
Nucleic Acids Res. 1985 Jun 11;13(11):4203-24 PMID: 2409533
Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element.
Mol Cell Biol. 1986 Jan;6(1):80-9 PMID: 3023839
Veal heart ribonuclease P has an essential RNA component.
Biochem Biophys Res Commun. 1980 Sep 30;96(2):831-7 PMID: 6158945
End labeling of enzymatically decapped mRNA.
Nucleic Acids Res. 1977 Dec;4(12):4165-74 PMID: 202926
Snurps and scyrps.
Cell. 1981 Aug;25(2):298-300 PMID: 6169438
Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains.
Proc Natl Acad Sci U S A. 1984 Jan;81(1):125-9 PMID: 16593404
Some simple computational methods to improve the folding of large RNAs.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):45-52 PMID: 6198623
An archaebacterial 5S rRNA contains a long insertion sequence.
Nature. 1981 Oct 29;293(5835):755-6 PMID: 6169998
The conformation of chicken, rat and human U1A RNAs in solution.
Nucleic Acids Res. 1981 Feb 25;9(4):841-58 PMID: 6164982
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
Cell. 1984 Aug;38(1):299-307 PMID: 6235919
Nucleolytic processing of a tRNAArg-tRNAAsp dimeric precursor by a homologous component from Saccharomyces cerevisiae.
J Biol Chem. 1985 Jan 25;260(2):1271-9 PMID: 2981839
In vitro processing of B. mori transfer RNA precursor molecules.
Cell. 1979 Jun;17(2):389-97 PMID: 378409
RNase P of Bacillus subtilis has a RNA component.
J Biol Chem. 1980 Aug 25;255(16):7507-9 PMID: 6156938
Reconstitution of RNAase P activity using inactive subunits from E. coli and HeLa cells.
Cell. 1986 Jan 31;44(2):243-9 PMID: 2417727
Replicative form of Semliki Forest virus RNA contains an unpaired guanosine.
Nature. 1979 Dec 13;282(5740):754-6 PMID: 514358
Ribonuclease P: an enzyme with an essential RNA component.
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3717-21 PMID: 358197
Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6475-9 PMID: 6292903
A catalytic RNA and its gene from Salmonella typhimurium.
Science. 1985 May 24;228(4702):999-1002 PMID: 2408335
Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.
J Biol Chem. 1982 Jul 25;257(14):8432-41 PMID: 7045122

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1986-07-00

Pages

1697-703

Language

English

Region

England

NLM ID

8208664

PMCID

PMC1166996

Subset

Databases

GENBANK

X04013

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.

Contact

WeChat Official Account