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PMID: 374994 Published · ppublish English Journal Article

The contruction and replication properties of hybrid plasmids composed of the r-determinant of R100.1 and the plasmids pCRI and pSC201.

Molecular & general genetics : MGG ·Vol. 168 ·No. 3 ·1979-01-11 ·Pages 337-40

Lane D, Chandler M, Silver L, Bruschi A, Caro L

Abstract

We have cloned the entire r-determinant of the antibiotic resistance plasmid R100.1 on the plasmic vectors pCR1 and pSC201. We find that the hybrid plasmids segregate from cultures in which replication of the vector is blocked. This suggests that the r-det is not capable of autonomous replication.

MeSH Terms
DNA Replication DNA Restriction Enzymes/genetics DNA, Recombinant Escherichia coli/genetics R Factors Temperature
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lane D
Chandler M
Silver L
Bruschi A
Caro L
References (17)
17 references, click to expand
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  3. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes.
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    Mol Gen Genet. 1977 Nov 29;157(1):17-23 PMID: 340913
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    Nat New Biol. 1971 Nov 10;234(45):40-3 PMID: 4942895
  9. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.
    J Bacteriol. 1972 Mar;109(3):971-8 PMID: 4551758
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    Mol Gen Genet. 1976 Feb 2;143(3):311-8 PMID: 765763
  14. Suppression of an Escherichia coli dnaA mutation by the integrated R factor R100.1: generation of small plasmids after integration.
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271505
Published
1979-01-11
Pages
337-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271505
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