Home LiteratureArticle Details
PMID: 3755817 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase.

Nucleic acids research ·Vol. 14 ·No. 15 ·1986-08-11 ·Pages 6053-66

Grant PM, Tellam J, May VL, Strauss AW

Abstract

We have determined the complete sequence of the rat mitochondrial malate dehydrogenase (mMDH) precursor derived from nucleotide sequence of the cDNA. A single synthetic oligodeoxynucleotide probe was used to screen a rat atrial cDNA library constructed in lambda gt10. A 1.2 kb full-length cDNA clone provided the first complete amino acid sequence of pre-mMDH. The 1014 nucleotide-long open reading frame encodes the 314 residue long mature mMDH protein and a 24 amino acid NH2-terminal extension which directs mitochondrial import and is cleaved from the precursor after import to generate mature mMDH. The amino acid composition of the transit peptide is polar and basic. The pre-mMDH transit peptide shows marked homology with those of two other enzymes targeted to the rat mitochondrial matrix.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA/genetics DNA, Recombinant/analysis Genes Malate Dehydrogenase/genetics Mitochondria/enzymology Protein Precursors/genetics Rats Sequence Homology, Nucleic Acid
Chemicals
DNA, Recombinant Protein Precursors DNA Malate Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grant P M
Tellam J
May V L
Strauss A W
References (27)
27 references, click to expand
  1. Different transport pathways of individual precursor proteins in mitochondria.
    Eur J Biochem. 1981 Jun 1;116(3):455-60 PMID: 6167439
  2. Targeting of pre-ornithine transcarbamylase to mitochondria: definition of critical regions and residues in the leader peptide.
    Cell. 1986 Feb 14;44(3):451-9 PMID: 3943133
  3. The primary translation product of rat intestinal apolipoprotein A-I mRNA is an unusual preproprotein.
    J Biol Chem. 1982 Jan 25;257(2):971-8 PMID: 6798036
  4. Rat liver L-glutamate dehydrogenase, malate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, and sulfite oxidase are each synthesized as larger precursors by cytoplasmic free polysomes.
    J Biol Chem. 1982 Apr 10;257(7):3355-8 PMID: 7061482
  5. Amino acid sequence homology among the 2-hydroxy acid dehydrogenases: mitochondrial and cytoplasmic malate dehydrogenases form a homologous system with lactate dehydrogenase.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6166-70 PMID: 6959107
  6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  7. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1358-62 PMID: 266178
  10. Prevention of NH2-terminal acetylation of proteins synthesized in cell-free systems.
    J Biol Chem. 1977 Dec 25;252(24):8781-3 PMID: 925022
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  13. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  14. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  15. How mitochondria import proteins.
    Biochim Biophys Acta. 1984 Jan 27;779(1):65-87 PMID: 6318829
  16. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  17. Isolation of the human insulin-like growth factor I gene using a single synthetic DNA probe.
    EMBO J. 1984 Feb;3(2):361-4 PMID: 6232133
  18. Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase.
    Science. 1984 Jun 8;224(4653):1068-74 PMID: 6372096
  19. Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4647-51 PMID: 6589615
  20. Isolation and characterization of a cDNA clone for bovine cytochrome c oxidase subunit IV.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6295-9 PMID: 6093095
  21. Import of rat liver mitochondrial malate dehydrogenase. Binding of the precursor to mitochondria, an intermediate step in import.
    J Biol Chem. 1984 Nov 25;259(22):13633-6 PMID: 6389531
  22. A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.
    EMBO J. 1985 May;4(5):1129-35 PMID: 3891325
  23. Cloning and sequence analysis of a cDNA encoding porcine mitochondrial aspartate aminotransferase precursor.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6065-9 PMID: 3862118
  24. Sequence of the precursor to rat ornithine aminotransferase deduced from a cDNA clone.
    J Biol Chem. 1985 Oct 25;260(24):12993-7 PMID: 3840476
  25. The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.
    EMBO J. 1985 Aug;4(8):2061-8 PMID: 2998781
  26. A synthetic signal peptide blocks import of precursor proteins destined for the mitochondrial inner membrane or matrix.
    J Biol Chem. 1985 Dec 25;260(30):16045-8 PMID: 4066702
  27. A putative precursor of rat liver mitochondrial malate dehydrogenase.
    FEBS Lett. 1981 Oct 12;133(1):127-9 PMID: 6171455
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.15.6053
Published
1986-08-11
Pages
6053-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311621
Subset
IM
Grants
NIADDK NIH HHS · AM-20407 · United States
NIGMS NIH HHS · GM-07200 · United States
Databases
GENBANK
X04240
External Links
PubMed source DOI: 10.1093/nar/14.15.6053 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2