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PMID: 3763406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chromatin structure of the promoter region of the human c-K-ras gene.

Nucleic acids research ·Vol. 14 ·No. 18 ·1986-09-25 ·Pages 7361-78

Jordano J, Perucho M

Abstract

The chromatin structure of the human c-K-ras gene has been investigated in various cultured normal and tumor human cells and in a rat cell line transformed with the human oncogene. The promoter region is hypersensitive to DNAse I, micrococcal nuclease, endogenous nucleases and to S1 nuclease in supercoiled plasmids. This hypersensitive region is present in the different cell types analyzed and both normal and mutant alleles exhibit similar general sensitivity to DNAse I digestion in the same tumor cells. However, the 5' more distal DNAse I hypersensitive site, which is coincident with a region of the gene containing sequence homologies with known enhancers, exhibits variable sensitivity which appears to be higher in the tumor than in the normal and in the human than in the rat cells which we have analyzed. These data suggest the presence of specific factors interacting with the promoter sequences and delimits the transcription unit of the c-K-ras locus.

MeSH Terms
Cell Line Cell Transformation, Neoplastic Chromatin/ultrastructure Deoxyribonuclease I/metabolism Humans Lung Neoplasms Micrococcal Nuclease Nucleic Acid Hybridization Oncogenes Plasmids Promoter Regions, Genetic Substrate Specificity
Chemicals
Chromatin Deoxyribonuclease I Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jordano J
Perucho M
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.18.7361
Published
1986-09-25
Pages
7361-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311756
Subset
IM
Grants
NCI NIH HHS · CA-33021 · United States
External Links
PubMed source DOI: 10.1093/nar/14.18.7361 PMC Full Text View journal details
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